Molecular cloning of a novel nuclear factor, TDRP1, in spermatogenic cells of testis and its relationship with spermatogenesis.
Molecular cloning of a novel nuclear factor, TDRP1, in spermatogenic cells of testis and its relationship with spermatogenesis.
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DOI：
10.1016/j.bbrc.2010.02.061
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发表时间：
2010-03
影响因子：
3.1
通讯作者：
Xuanchun Wang;Haowen Jiang;Wenbai Zhou;Zhaoyun Zhang;Zhihong Yang;Yong Lu;B. Lu;Xiang Wang;Q. Ding;R. Hu
中科院分区：
文献类型：
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作者：
Xuanchun Wang;Haowen Jiang;Wenbai Zhou;Zhaoyun Zhang;Zhihong Yang;Yong Lu;B. Lu;Xiang Wang;Q. Ding;R. Hu
我们报道了一个可能参与精子发生的名为TDRP（编码睾丸发育相关蛋白）的新基因的鉴定。人类TDRP基因有两个不同的转录本，TDRP1和TDRP2，分别编码183 aa和198 aa的蛋白。Tdrp mRNA主要在睾丸组织中表达。制备了兔抗人TDRP1特异性多克隆抗体。免疫组化分析显示，TDRP1在生精细胞中表达，尤其是在精母细胞中高表达。我们提供的证据表明，TDRP1分布在生精细胞的细胞质和细胞核中。研究了Tdrp1 mRNA及其蛋白在不同发育阶段大鼠睾丸组织中的表达规律。Tdrp1 mRNA及其蛋白在新生大鼠睾丸中几乎没有检测到，产后3周显著升高，产后2个月达到峰值。我们还研究了TDRP1在精子发生缺陷的无精子男性睾丸组织中的表达。Western blot分析显示，与正常对照相比，无精子症男性睾丸组织中TDRP1的表达明显降低。这些目前的数据表明，作为一个核因子，TDRP1可能在精子发生中发挥重要作用。
We reported the identification of a novel gene termed TDRP (encoding testis development-related protein) that might be involved in spermatogenesis. The human TDRP gene had two distinct transcripts, TDRP1 and TDRP2, which encoded proteins of 183 aa and 198 aa respectively. Tdrp mRNA was predominantly expressed in testis tissue. We generated rabbit polyclonal antibodies specific against human TDRP1. Immunohistochemistry analysis showed TDRP1 was expressed in spermatogenic cells, especially with high expression in spermatocytes. We provided evidence that TDRP1 distributed in both cytoplasm and nuclei of spermatogenic cells. Expression patterns of Tdrp1 mRNA and its protein were investigated in the rat testis tissues of different developmental stages. Both Tdrp1 mRNA and its protein were barely detected in the testis of neonatal rats, increased remarkably at 3weeks postpartum, and peaked at 2months postpartum. We also investigated TDRP1 expressions in testis tissues of azoospermic men with defective spermatogenesis. Western blot analysis showed that TDRP1 expressions were significantly lower in the testis tissues of azoospermic men compared with normal controls. These current data demonstrated that as a nuclear factor, TDRP1 might play an important role in spermatogenesis.