Immunocytochemical Localization of the Translocase of the Outer Mitochondrial Membrane (Tom20) in the Human Cochlea
Immunocytochemical Localization of the Translocase of the Outer Mitochondrial Membrane (Tom20) in the Human Cochlea
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DOI：
10.1002/ar.22622
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发表时间：
2013-02-01
影响因子：
2
通讯作者：
Ishiyama, Akira
中科院分区：
文献类型：
--
作者：
Balaker, Ashley E.;Ishiyama, Paul;Ishiyama, Akira
内耳的线粒体退化可能是与年龄相关的听力损失和其他耳部疾病(如梅尼埃病)的一个促成因素。大多数线粒体蛋白是在胞浆中合成的，并通过转运子通过线粒体膜输入。外膜转位酶(TOM)是所有进入线粒体的蛋白质的通用进入门。转运体功能的改变可能会改变蛋白质进入线粒体的运输，并扰乱功能。在这项研究中，我们确定了主要的线粒体蛋白输入受体Tom20在显微解剖的人耳蜗冰冻切片和火棉胶包埋的档案标本中的免疫定位。我们用亲和纯化的兔抗Tom20多克隆抗体。我们还测定了Tom20在小鼠内耳的免疫定位。在人类和小鼠的耳蜗中，Tom20普遍分布在Corti器官中，可以很好地显示内毛细胞和外毛细胞。Tom20免疫反应定位于螺旋神经节神经元胞浆。在老年梅尼埃病患者内耳中，Tom20的表达减少。这些结果表明，Tom20基因可以在内耳中作为线粒体蛋白输入的标志。阿纳特·雷克，2013。(C)2012年威利期刊公司。
Mitochondrial degeneration in the inner ear is likely a contributing factor in age-related hearing loss and other otopathologies such as Meniere's disease. Most mitochondrial proteins are synthesized in the cytosol and imported through the mitochondrial membranes by translocators. The translocase of the outer membrane (Tom) is the universal entry gate for all proteins that are imported into mitochondria. Altered function of the translocator could alter protein transport into the mitochondria, and disrupt function. In this study, we determined the immunolocalization of Tom20, a major mitochondrial protein import receptor, in microdissected human cochlea frozen sections obtained from postmortem autopsy and celloidin-embedded archival specimens. We used affinity purified rabbit polyclonal antibodies against Tom20. We also determined the Tom20 immunolocalization in the mouse inner ear. In the human and mouse cochlea, Tom20 was ubiquitously distributed in the organ of Corti, allowing well-delineated visualization of inner and outer hair cells. Tom20 immunoreactivity localized in the cytoplasm of spiral ganglia neurons. In the inner ear of aged subjects with Meniere's disease, there was decreased expression of Tom20. These results suggest that Tom20 can be used in the inner ear as a marker for mitochondrial protein import. Anat Rec, 2013. (c) 2012 Wiley Periodicals, Inc.