Differential expression of plasminogen activator inhibitor-1, tumor necrosis factor-α, TNF-α converting enzyme and ADAMTS family members in murine fat territories
Differential expression of plasminogen activator inhibitor-1, tumor necrosis factor-α, TNF-α converting enzyme and ADAMTS family members in murine fat territories
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DOI：
10.1016/s0167-4781(02)00589-4
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发表时间：
2003-01-03
期刊：
影响因子：
--
通讯作者：
Lijnen, HR
中科院分区：
文献类型：
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作者：
Voros, G;Maquoi, E;Lijnen, HR
我们的目的是研究去整合素和金属蛋白酶（ADAM）和具有血小板反应蛋白（TS）基序的ADAM蛋白（ADAMTS）家族成员在瘦小鼠和肥胖小鼠脂肪组织中的表达。将5周龄的雄性小鼠保持标准食物（SFD）或高脂肪饮食（HFD）15周，收获皮下（SC）和性腺（GON）脂肪组织以及成熟脂肪细胞和基质血管（S-V）细胞。在分离的脂肪组织和细胞组分中以及在鼠前脂肪细胞分化期间定量纤溶酶原激活物抑制剂-1（派-1）、肿瘤坏死因子-α（TNF-α）、ADAM-17 TACE或TNF-α转化酶）、ADAMTS-1和ADAMTS-8的mRNA水平。HFD导致分离的SC和GON脂肪垫的重量显著增加，并且葡萄糖、胆固醇和派-1的血液水平增加。在瘦小鼠（SFD）的SC和GON脂肪组织中均检测到ADAM-17、TNF-α、派-1、ADAMTS-1和ADAMTS-8 mRNA。在肥胖小鼠（HFD）的SC脂肪组织中，ADAM-17和派-1的表达增强，ADAMTS-1的表达降低，而在GON脂肪组织中，TNF-α的表达增强，ADAMTS-8的表达降低。在瘦和肥胖小鼠中，ADAM-17、ADAMTS-1和ADAMTS-8在S-V细胞组分中的表达高于成熟脂肪细胞。在小鼠3 T3-F442 A前脂肪细胞的分化过程中，ADAM-17和ADAMTS-1的表达几乎保持不变，而ADAMTS-8的表达随着脂肪细胞的成熟而下降。几种ADAM和ADAMTS家族成员在脂肪组织中和前脂肪细胞分化期间表达。它们的表达在肥胖发展时的调节是脂肪组织依赖性的。(C)2002 Elsevier Science B. V.保留所有权利。
Our objective was to investigate expression of A disintegrin and metalloproteinase (ADAM) and ADAM proteins with a thrombospondin (TS) motif (ADAMTS) family members in adipose tissue of lean and obese mice. Five-week-old male mice were kept on standard chow (SFD) or on high fat diet (HFD) for 15 weeks, and subcutaneous (SC) and gonadal (GON) adipose tissue, as well as mature adipocytes and stromal-vascular (S-V) cells were harvested. mRNA levels of plasminogen activator inhibitor-1 (PAI-1), tumor necrosis factor-alpha (TNF-alpha), ADAM-17 TACE or TNF-alpha converting enzyme), ADAMTS-1 and ADAMTS-8 were quantified in isolated adipose tissues and cell fractions, and during differentiation of murine preadipocytes. The HFD resulted in a significantly enhanced weight of isolated SC and GON fat pads, and in enhanced blood levels of glucose, cholesterol and PAI-1. ADAM-17, TNF-alpha, PAI-1, ADAMTS-1 and ADAMTS-8 mRNA were detected in both SC and GON adipose tissue of lean mice (SFD). In SC adipose tissue of obese mice (HFD), the expression of ADAM-17 and PAI-1 was enhanced and that of ADAMTS-1 reduced, whereas in GON adipose tissue expression of TNF-alpha was enhanced and that of ADAMTS-8 reduced. In lean and obese mice, expression of ADAM-17, ADAMTS-1 and ADAMTS-8 was higher in the S-V cell fraction than in mature adipocytes. During differentiation of murine 3T3-F442A preadipocytes, expression of ADAM-17 and ADAMTS-1 remained virtually unaltered, whereas that of ADAMTS-8 decreased as adipocytes matured. Several ADAM and ADAMTS family members are expressed in adipose tissue and during differentiation of preadipocytes. Modulation of their expression upon development of obesity is adipose tissue-dependent. (C) 2002 Elsevier Science B.V. All rights reserved.