Quantitative analysis of estimated scattering coefficient and phase retardation for ovarian tissue characterization.
Quantitative analysis of estimated scattering coefficient and phase retardation for ovarian tissue characterization.
复制标题
DOI：
10.1364/boe.3.001548
复制
发表时间：
2012-07-01
影响因子：
3.4
通讯作者：
Zhu Q
中科院分区：
文献类型：
--
作者：
Yang Y;Wang T;Wang X;Sanders M;Brewer M;Zhu Q
在本报告中，通过偏振敏感 OCT (PSOCT) 定量估计的光学散射系数和相位延迟用于卵巢组织表征。对从 18 名患者获得的总共 33 个离体卵巢（正常：n = 26，恶性：n = 7）进行了研究。仅使用估计的散射系数即可实现 100% 的特异性和 86% 的灵敏度；单独使用相位延迟获得了100%的特异性和43%的灵敏度。然而，如果将这两个参数一起用于正常和恶性卵巢的分类，则可以实现 100% 的优异特异性和 100% 的敏感性。从天狼星红组织学切片获得的胶原蛋白含量的定量测量表明，它与估计的散射系数和相位延迟相关。我们的初步结果表明，PSOCT 的定量分析可能是微创手术期间区分正常和恶性卵巢组织的潜在有价值的方法，并有助于指导手术干预。
In this report, optical scattering coefficient and phase retardation quantitatively estimated from polarization-sensitive OCT (PSOCT) were used for ovarian tissue characterization. A total of 33 ex vivo ovaries (normal: n = 26, malignant: n = 7) obtained from 18 patients were investigated. A specificity of 100% and a sensitivity of 86% were achieved by using estimated scattering coefficient alone; and a specificity of 100% and a sensitivity of 43% were obtained by using phase retardation alone. However, a superior specificity of 100% and sensitivity of 100% were achieved if these two parameters were used together for classifying normal and malignant ovaries. Quantitative measurement of collagen content obtained from Sirius red histology sections shows that it correlates with estimated scattering coefficient and phase retardation. Our initial results demonstrate that quantitative analysis of PSOCT could be a potentially valuable method for distinguishing normal from malignant ovarian tissues during minimally invasive surgery and help guide surgical intervention.