Photonic detection of bacterial pathogens in living hosts
Photonic detection of bacterial pathogens in living hosts
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DOI：
10.1111/j.1365-2958.1995.mmi_18040593.x
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发表时间：
1995-11-01
影响因子：
3.6
通讯作者：
Benaron, DA
中科院分区：
文献类型：
--
作者：
Contag, CH;Contag, PR;Benaron, DA
致病过程的研究通常限于离体测定和细胞培养相关物。体内分析将有助于更好地了解传染病。因此，我们已经开发了一种用于检测活宿主中的细菌病原体的方法，并使用该方法来评估鼠伤寒沙门氏菌菌株对小鼠的毒力不同的疾病过程。通过用赋予细菌荧光素酶组成型表达的质粒转化，用生物发光标记三种沙门氏菌菌株。检测通过感染生物发光沙门氏菌的动物组织传输的光子，可以将细菌定位到特定组织。以这种方式，进行性感染与持续性或流产性感染区分开来。我们观察到的生物发光模式表明盲肠可能在沙门氏菌的发病机制中发挥关键作用。使用该光学方法监测抗生素的体内功效。这项研究表明，真实的时间无创分析的病原体事件和药理学监测可以在体内进行。
The study of pathogenic processes is often limited to ex vivo assays and cell-culture correlates. A greater understanding of infectious diseases would be facilitated by in vivo analyses. Therefore, we have developed a method for detecting bacterial pathogens in a living host and used this method to evaluate disease processes for strains of Salmonella typhimurium that differ in their virulence for mice. Three strains of Salmonella were marked with bioluminescence through transformation with a plasmid conferring constitutive expression of bacterial luciferase. Detection of photons transmitted through tissues of animals infected with bioluminescent Salmonella allowed localization of the bacteria to specific tissues. In this manner progressive infections were distinguished from those that were persistent or abortive. We observed patterns of bioluminescence that suggested the caecum may play a pivotal role in Salmonella pathogenesis. in vivo efficacy of an antibiotic was monitored using this optical method. This study demonstrates that real time noninvasive analyses of pathogenic events and pharmacological monitoring can be performed in vivo.