Increased DNA methyltransferase 1 protein expression in human transitional cell carcinoma of the bladder
Increased DNA methyltransferase 1 protein expression in human transitional cell carcinoma of the bladder
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DOI：
10.1097/01.ju.0000095919.50869.c9
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发表时间：
2003-12-01
影响因子：
6.6
通讯作者：
Hirohashi, S
中科院分区：
文献类型：
--
作者：
Nakagawa, T;Kanai, Y;Hirohashi, S
目的：DNA甲基化是基因转录和基因组稳定性的关键调控因子，DNA甲基化的改变是人类癌症中最一致的表观遗传变化之一。我们阐明了DNA甲基转移酶（DNMT） 1（一种参与基因组甲基化模式测定的主要酶）在人类尿路上皮癌发生过程中异常蛋白表达的意义。材料与方法：正常尿路上皮61例，非癌性尿路上皮89例。对膀胱癌（nbc）患者、78例发育不良尿路上皮患者和174例移行细胞癌（tcc）患者进行DNMT1免疫组化分析。结果：核DNMT1免疫反应性在NBCs中的发生率（65%）明显高于正常尿路上皮的发生率(20%,p
Purpose: DNA methylation is a key regulator of gene transcription and genomic stability, and alteration of DNA methylation is one of the most consistent epigenetic changes in human cancers. We elucidated the significance of aberrant protein expression of DNA methyltransferase (DNMT) 1, a major enzyme involved in the determination of genomic methylation patterns, during human urothelial carcinogenesis.Materials and Methods: A total of 61 samples of normal urothelium, 89 noncancerous urothelium. samples showing no remarkable histological changes from patients with bladder cancer (NBCs), 78 dysplastic urothelium samples and 174 transitional cell carcinoma samples (TCCs) were subjected to immunohistochemical analysis for DNMT1.Results: The incidence of nuclear DNMT1 immunoreactivity in NBCs (65%) was significantly higher than in normal urothelium (20%, p