Cultivation of roots in bioreactors
Cultivation of roots in bioreactors
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DOI：
10.1016/0958-1669(93)90126-h
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发表时间：
1993-01-01
影响因子：
7.7
通讯作者：
Curtis, Wayne R.
中科院分区：
文献类型：
--
作者：
Curtis, Wayne R.
在过去的几年里，在反应器中培养根的报道数量急剧增加。这提供了培养根的物理和代谢要求的急需的信息，并建立了根组织的操作技术。然而，大多数这项工作都是在相对较小的规模（小于5升）上进行的，许多都是示范，而不是系统的研究。规模扩大的真实的挑战将在向100-1000 l规模发展时遇到，同时保持干组织密度超过10-20（g干重）l-1。要实现这一目标，就需要进行小规模研究，重点是获得设计和操作信息，同时测量大规模系统的基本放大参数。
Over the past few years, the number of reports of roots cultured in reactors has increased dramatically. This has provided much-needed information on the physical and metabolic requirements of cultured roots, and has established techniques for the manipulation of root tissue. Most of this work, however, is conducted on a relatively small scale (less than 5 l), and many are demonstrations as opposed to systematic studies. The real challenge of scale-up will be encountered when moving to the 100–1000 l scale, while maintaining dry tissue densities in excess of 10–20 (g dry weight) l−1. Achievement of this goal will require small-scale studies, focused on obtaining design and operational information, coupled with measurements of fundamental scale-up parameters in large-scale systems.