Muscle specific versus ubiquitous expression of Gag based HIV-1 DNA vaccines: a comparative analysis
Muscle specific versus ubiquitous expression of Gag based HIV-1 DNA vaccines: a comparative analysis
复制标题
DOI：
10.1016/s0264-410x(02)00081-6
复制
发表时间：
2002-05-06
期刊：
影响因子：
5.5
通讯作者：
Wagner, R
中科院分区：
文献类型：
--
作者：
Bojak, A;Hammer, D;Wagner, R
大多数基于dna的免疫研究都使用病毒启动子来驱动抗原表达。在这项研究中，我们在体外分析了常用的CMV启动子、组织特异性小鼠肌酸激酶（MCK）启动子以及MCK/CMV混合启动子在启动子活性和组织特异性方面的特性。此外，我们还比较了肌肉免疫含有密码子优化合成基因的DNA后，在体内诱导HIV-1 Gag特异性免疫反应的效率。尽管与传统的基于巨细胞病毒的载体模块相比，使用MCK构建的抗体滴度和细胞免疫反应略有降低，但使用非病毒启动子可能会显著提高未来DNA疫苗的安全性。(C) 2002 Elsevier Science Ltd.版权所有。
Most studies on DNA-based immunization have used viral promoters to drive antigen expression. In this study, we analyzed the properties of the commonly used CMV promoter, the tissue specific murine muscle creatine kinase (MCK) promoter as well as a hybrid MCK/CMV promoter regarding promoter activity and tissue specificity in vitro. Furthermore, the efficiency of inducing HIV-1 Gag specific immune responses in vivo following intramuscular immunization of nacked DNA containing a codon optimized synthetic gene was compared. Although antibody titers and cellular immune responses using the MCK construct were slightly reduced as compared to convential CMV based vector modules, the utilization of nonviral promoters may add significantly to the safety of future DNA vaccines. (C) 2002 Elsevier Science Ltd. All rights reserved.