The effect of chitin and chitosan on the proliferation of human skin fibroblasts and keratinocytes in vitro
The effect of chitin and chitosan on the proliferation of human skin fibroblasts and keratinocytes in vitro
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DOI：
10.1016/s0142-9612(01)00042-4
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发表时间：
2001-11-01
期刊：
影响因子：
14
通讯作者：
Wood, EJ
中科院分区：
文献类型：
--
作者：
Howling, GI;Dettmar, PW;Wood, EJ
体外研究了几丁质[(1→4)-2-乙酰氨基-2-脱氧-β-D-葡聚糖]及其部分脱乙酰衍生物壳聚糖对人真皮成纤维细胞和角质形成细胞增殖的影响。脱乙酰度相对较高的壳聚糖强烈刺激成纤维细胞增殖，而脱乙酰度较低的样品则表现出较低的活性。使用来自一系列人类供体的成纤维细胞培养物进一步评估了级分 CL313A（链长较短、89% 脱乙酰氯化壳聚糖）。一些成纤维细胞培养物对 CL313A 处理产生积极的促有丝分裂反应，在 50 微克/毫升的初始浓度下，增殖率比对照水平增加约 50%，而其他培养物则没有显示出对增殖的刺激，甚至有轻微的抑制作用。
The effects of chitin [(1 -->4)-2-acetamido-2-deoxy-beta -D-glucan] and its partially deacetylated derivatives, chitosans, on the proliferation of human dermal fibroblasts and keratinocytes were examined in vitro. Chitosans with relatively high degrees of deacetylation strongly stimulated fibroblast proliferation while samples with lower levels of deacetylation showed less activity. Fraction, CL313A, a shorter chain length, 89% deacetylated chitosan chloride was further evaluated using cultures of fibroblasts derived from a range of human donors. Some fibroblast cultures produced a positive mitogenic response to CL313A treatment with proliferation rates being increased by approximately 50% over the control level at an initial concentration of 50 mug/ml, whilst others showed no stimulation of proliferation or even a slight inhibition (