Enhancing expression of SSU1 genes in Saccharomyces uvarum leads to an increase in sulfite tolerance and a transcriptome profile change
Enhancing expression of SSU1 genes in Saccharomyces uvarum leads to an increase in sulfite tolerance and a transcriptome profile change
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增强葡萄汁酵母中 SSU1 基因的表达导致亚硫酸盐耐受性增强和转录组谱变化
DOI：
10.1093/femsyr/fox023
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发表时间：
2017-05-01
影响因子：
3.2
通讯作者：
Zhang, Z. M.
中科院分区：
文献类型：
--
作者：
Liu, X. Z.;Sang, M.;Zhang, Z. M.
&NA;葡萄汁酵母（Saccharomyces uvarum）是一种良好的葡萄酒酵母菌种，未来可能具有巨大的潜力。然而，大多数葡萄球菌菌株的硫耐受性很差。此外，关于葡萄球菌 SSU1 基因的信息仍然很少，该基因编码一种假定的赋予亚硫酸盐耐受性的转运蛋白。为了分析SSU1基因的功能，构建了两个包含不同SSU1基因的表达载体，并将其转移到耐亚硫酸盐葡萄球菌菌株A9中。然后使用亚硫酸盐耐受性、SO2 产生以及 PCR、测序、RT-qPCR 和转录组分析来了解 S. uvarum SSU1 基因的功能。我们的结果表明，增强 SSU1 基因的表达可以促进 S. uvarum 的亚硫酸盐抗性，并且如在某些等位基因中所见，附加 SSU1 基因之前的插入片段可能会影响其他基因的表达和 S. uvarum 的亚硫酸盐耐受水平。这是关于增强葡萄球菌 SSU1 基因表达的第一份报告。图形抽象图。 SSU1 基因表达的增强可促进葡萄汁酵母的亚硫酸盐抗性，并且在 SSU1 基因的额外拷贝上游插入 DNA 片段会影响其他基因的表达和葡萄汁酵母的亚硫酸盐耐受水平。数字。没有可用的字幕。
&NA; Saccharomyces uvarum is a good wine yeast species that may have great potential for the future. However, sulfur tolerance of most S. uvarum strains is very poor. In addition there is still little information about the SSU1 gene of S. uvarum, which encodes a putative transporter conferring sulfite tolerance. In order to analyze the function of the SSU1 gene, two expression vectors that contained different SSU1 genes were constructed and transferred into a sulfite‐tolerant S. uvarum strain, A9. Then sulfite tolerance, SO2 production, and PCR, sequencing, RT‐qPCR and transcriptome analyses were used to access the function of the S. uvarum SSU1 gene. Our results illustrated that enhancing expression of the SSU1 gene can promote sulfite resistance in S. uvarum, and an insertion fragment ahead of the additional SSU1 gene, as seen in some alleles, could affect the expression of other genes and the sulfite tolerance level of S. uvarum. This is the first report on enhancing the expression of the SSU1 gene of S. uvarum. Graphical Abstract Figure. Enhanced expression of the SSU1 gene promotes sulfite resistance in Saccharomyces uvarum, and an insertion of a DNA fragment upstream of an additional copy of the SSU1 gene affects the expression of other genes and the sulfite tolerance level of S. uvarum. Figure. No caption abailable.