Molecular characterization of DNAβ molecules associated with Tobacco leaf curl Yunnan virus
Molecular characterization of DNAβ molecules associated with Tobacco leaf curl Yunnan virus
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DOI：
10.1111/j.1439-0434.2004.00911.x
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发表时间：
2004-12-01
影响因子：
1.5
通讯作者：
Zhou, XP
中科院分区：
文献类型：
--
作者：
Cui, XF;Xie, Y;Zhou, XP
云南卷叶病毒 (TbLCYNV) 分离株 Y136 和 Y143 均含有 DNAbeta 分子。克隆并表征了七个 DNAbeta 分子，每个分子由 1334-1341 个核苷酸组成。序列比较显示它们有两种主要类型：1型包括5个DNAbeta分子，总体核苷酸序列同一性为75-99%，2型包括2个DNAbeta分子，总体核苷酸序列一致性>99%。两种类型的 DNAbeta 分子之间仅发现 52-54% 的核苷酸同一性。这两种分离株均与两种类型的 DNAbeta 相关。关系树图分析显示，1型DNAβ分子与中国番茄黄曲叶病毒的DNAβ分子聚集在一起，而2型DNAβ分子与泰国番茄黄曲叶病毒的DNAβ聚集在一起。还有证据表明，2 型 DNAβ 分子可能是由烟草卷枝病毒的 DNAβ 与另一种未鉴定病毒的 DNAβ 重组而产生的。迄今为止，仅发现一种类型的 DNAbeta 分子与一种 Begomovirus 相关。讨论了TbLCYNV与两种类型的DNAβ分子相关的可能原因。
Tobacco leaf curl Yunnan virus (TbLCYNV) isolates Y136 and Y143 were both shown to contain DNAbeta molecules. Seven DNAbeta molecules, each consisting of 1334-1341 nucleotides, were cloned and characterized. Sequence comparison showed that they were of two main types: type 1 included five DNAbeta molecules with 75-99% overall nucleotide sequence identity, and type 2 included two DNAbeta molecules with >99% overall nucleotide sequence identity. Only 52-54% nucleotide identity was found between the two types of DNAbeta molecules. The two isolates were associated with both types of DNAbeta. A relationship dendrogram analysis showed that type 1 DNAbeta molecules clustered with the DNAbeta molecules of Tomato yellow leaf curl China virus, whereas type 2 DNAbeta molecules clustered with the DNAbeta of Tomato yellow leaf curl Thailand virus. Evidence was also provided that type 2 DNAbeta molecules may have arisen by recombination between the DNAbeta of Tobacco curly shoot virus and another unidentified virus. To date, only a single type of DNAbeta molecule had been found to be associated with one begomovirus. The possible reasons of TbLCYNV associated with two types of DNAbeta molecules are discussed.