Experimental manipulation of the microbial functional amyloid called curli.
Experimental manipulation of the microbial functional amyloid called curli.
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DOI：
10.1007/978-1-62703-245-2_4
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Chapman, Matthew R
中科院分区：
文献类型：
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作者：
Zhou, Yizhou;Smith, Daniel R;Chapman, Matthew R
卷曲是许多革兰氏阴性细菌表面产生的蛋白质纤维结构。作为细胞外基质的主要成分，curli介导细菌与其环境之间的相互作用，因此，curli在生物膜形成中起关键作用。卷曲纤维与越来越多的非常稳定和有序的蛋白质聚集体（称为淀粉样蛋白）共享生物物理特性。在这里，我们描述的实验方法来研究生物起源和大会curli利用其淀粉样蛋白的性质。我们还提出了分析卷曲介导的生物膜形成的方法。这些方法很简单，可以很容易地适用于研究其他细菌产生的淀粉样蛋白。
Curli are proteinaceous fibrous structures produced on the surface of many gram-negative bacteria. As a major constituent of the extracellular matrix, curli mediate interactions between the bacteria and its environment, and as such, curli play a critical role in biofilm formation. Curli fibers share biophysical properties with a growing number of remarkably stable and ordered protein aggregates called amyloid. Here we describe experimental methods to study the biogenesis and assembly of curli by exploiting their amyloid properties. We also present methods to analyze curli-mediated biofilm formation. These approaches are straightforward and can easily be adapted to study other bacterially produced amyloids.