Carbon nanotubes as scaffolds for cell culture and effect on cellular functions
Carbon nanotubes as scaffolds for cell culture and effect on cellular functions
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DOI：
10.4012/dmj.26.178
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发表时间：
2007-03-01
影响因子：
2.5
通讯作者：
Yokoyama, Atsuro
中科院分区：
文献类型：
--
作者：
Aoki, Naofumi;Akasaka, Tsukasa;Yokoyama, Atsuro
为了研究碳材料的相容性对晶体结构的依赖性，以开发生物医学应用为目的，采用单壁（SW）和多壁（MW）碳纳米管（CNTs）作为细胞培养的支架，并与石墨（GP）进行了比较。利用SaOS2细胞研究成骨样细胞的特性和反应。聚碳酸酯膜经真空过滤包被碳纳米管，形成网状的纳米结构。在CNTs上生长的细胞向各个方向伸展。在细胞增殖、碱性磷酸酶（ALP）活性和蛋白质在底物上的吸附方面，CNTs表现出比PC和GP更好的效果。SW的细胞增殖和总ALP表现最好。这些有利结果可能归因于碳纳米管的结构和碳纳米管对蛋白质的亲和力，从而表明碳纳米管可能是潜在的细胞培养支架材料。
To investigate the dependence of blocompatibility of carbon materials on crystal structure with the aim of developing biomedical applications, single-(SW) and multi-walled (MW) carbon nanotubes (CNTs) were employed as scaffolds for cell culture and compared with graphite (GP). SaOS2 cells were used to investigate the properties and response of osteoblast-like cells. Polycarbonate membranes (PC) coated with CNTs by vacuum filtration formed a meshwork nanostructure. Cells grown on CNTs greatly extended in all directions. In terms of cell proliferation, alkaline phosphatase (ALP) activity, and protein adsorption on the substrates, CNTs showed better results than PC and GP. SW showed the best cell proliferation and total ALP. These favorable results might be attributed to the structure of CNTs and the affinity of CNTs toward proteins, thereby suggesting that CNTs could be potential scaffold materials for cell culture.