Anti-inflammatory and anti-arthritic effects of Guge Fengtong Formula: in vitro and in vivo studies.
Anti-inflammatory and anti-arthritic effects of Guge Fengtong Formula: in vitro and in vivo studies.
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DOI：
10.1016/s1875-5364(15)30088-1
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发表时间：
2015-11-01
影响因子：
4.6
通讯作者：
Liu, E-Hu
中科院分区：
文献类型：
--
作者：
Cheng, Xiao-Lan;Liu, Xin-Guang;Liu, E-Hu
类风湿性关节炎（RA）是最常见的炎性关节炎，也是导致残疾的主要原因。目前，临床治疗炎症和关节炎疾病的药物因不良反应严重或无效而不尽如人意。古格风痛方（GGFT）含有山药、鸡血藤和姜的标准化提取物，长期以来被中国中医用于治疗类风湿性关节炎。然而，GGFT的详细抗炎和抗关节炎活性迄今尚未见报道。在本工作中，我们旨在利用三种体内动物模型评估GGFT的抗炎和抗关节炎作用，并试图初步揭示其在RAW 264.7巨噬细胞中的潜在作用机制。所得结果表明，GGFT 显着减轻耳部水肿，减少角叉菜胶诱导的足部水肿，降低关节炎评分，并逆转注射完全弗氏佐剂 (CFA) 的大鼠的体重减轻。此外，在 GGFT 治疗的大鼠中观察到关节滑膜炎症浸润和滑膜内层增生显着减少，血清中炎症因子（TNF-α 和 IL-1β）下降。在脂多糖激活的RAW264.7巨噬细胞中，GGFT减少NO、PGE2和IL-6的产生，并抑制iNOS、COX-2和NF-κB的表达。我们的研究结果表明，GGFT具有相当大的抗炎活性，对佐剂诱导的大鼠关节炎具有潜在的治疗作用，这为其传统应用治疗RA和其他炎症性疾病提供了实验证据。
Rheumatoid arthritis (RA) is the most common inflammatory arthritis and a major cause of disability. Presently, the clinical therapeutic medicines for inflammatory and arthritic diseases are unsatisfactory due to severe adverse effects or ineffectiveness. The Guge Fengtong formula (GGFT), containing the standardized extracts of Dioscoreae Nipponicae Rhizoma, Spatholobi Caulis, and Zingiberis Rhizoma, has long been used for RA treatment by Chinese doctorsin China. However, the detailed anti-inflammatory and anti-arthritic activity of GGFT has not been reported so far. In the present work, we aimed to evaluate the anti-inflammatory and anti-arthritic effects of GGFT using three in vivo animal models, and tried to uncover its preliminarythe underlying mechanism of action mechanism in RAW 264.7 macrophages. The obtained results indicated that GGFT significantly attenuated ear edema, decreased carrageenan-induced paw edema, reduced the arthritis score, and reversed the weight loss of the complete Freund's adjuvant (CFA)CFA-injected rats. Additionally, marked decrease of in synovial inflammatory infiltration and synovial lining hyperplasia in the joints and decline of inflammatory factors (TNF-alpha and IL-1beta) in the serum were observed in the GGFT-treated rats. In lipopolysaccharide-activated RAW264.7 macrophages, GGFT reduced the production of NO, PGE2, and IL-6, and inhibited the expression of iNOS, COX-2, and NF-kappaB expression. Our results demonstrated that GGFT possessed considerable anti-inflammatory activity and have had potential therapeutic effects on adjuvant induced arthritis in rats, which provided providing experimental evidences for its traditional application in the treatment of RA and other inflammatory diseases.