A complex containing PGRL1 and PGR5 is involved in the switch between linear and cyclic electron flow in Arabidopsis
A complex containing PGRL1 and PGR5 is involved in the switch between linear and cyclic electron flow in Arabidopsis
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DOI：
10.1016/j.cell.2007.12.028
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发表时间：
2008-01-25
期刊：
影响因子：
64.5
通讯作者：
Leister, Dario
中科院分区：
文献类型：
--
作者：
DalCorso, Giovanni;Pesaresi, Paolo;Leister, Dario
在光合作用过程中，位于叶绿体类囊体膜上的两个光反应中心，光系统I和光系统II(PSI和PSII)，利用光能动员电子产生ATP和NADPH。存在不同的电子流模式，其中线性电子流由PSI和PSII驱动，产生ATP和NADPH，而循环电子流(CEF)只产生ATP，由PSI单独驱动。不同的环境和代谢条件要求调整ATP/NADPH的比例，并在两个光系统之间切换电子分布。除PGR5外，其他促进CEF的成分尚不清楚。在这里，我们报告了一种存在于拟南芥类囊体中的跨膜蛋白PGRL1的鉴定。缺乏PGRL1的植株表现出CEF的扰动，类似于缺乏PGR5的植株。我们发现，PGRL1和PGR5在物理上相互作用，并与PSI相关。因此，我们认为PGRL1-PGR5复合体促进了真核生物的CEF。
During photosynthesis, two photoreaction centers located in the thylakoid membranes of the chloroplast, photosystems I and II (PSI and PSII), use light energy to mobilize electrons to generate ATP and NADPH. Different modes of electron flow exist, of which the linear electron flow is driven by PSI and PSII, generating ATP and NADPH, whereas the cyclic electron flow (CEF) only generates ATP and is driven by the PSI alone. Different environmental and metabolic conditions require the adjustment of ATP/NADPH ratios and a switch of electron distribution between the two photosystems. With the exception of PGR5, other components facilitating CEF are unknown. Here, we report the identification of PGRL1, a transmembrane protein present in thylakoids of Arabidopsis thaliana. Plants lacking PGRL1 show perturbation of CEF, similar to PGR5-deficient plants. We find that PGRL1 and PGR5 interact physically and associate with PSI. We therefore propose that the PGRL1-PGR5 complex facilitates CEF in eukaryotes.