Effects of cold treatment on synchronous Chinese hamster cells treated in mitosis
Effects of cold treatment on synchronous Chinese hamster cells treated in mitosis
复制标题
冷处理对同步中国仓鼠细胞有丝分裂的影响
DOI：
10.1002/jcp.1040800111
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发表时间：
1972
影响因子：
5.6
通讯作者：
W. Dewey
中科院分区：
文献类型：
--
作者：
H. Nagasawa;W. Dewey
从单层异步培养物中振摇中国仓鼠有丝分裂细胞（有丝分裂95%，中期85%，后期10%），并在1 ℃下储存长达28小时。在此期间，有丝分裂指数没有降低，细胞保持细胞学正常。然而，在4小时内，位于后期1.8分钟内的中期细胞（占中期细胞群的10%）能够进入后期; 1.8分钟的时间点与纺锤体激活标记物报告的时间点一致。当细胞升温至37°C时，它们进入G1和S的时间延迟（处理4小时后分别为35和70分钟）。这种70分钟的延迟维持了三个细胞周期，在此期间保持了高度的同步。冷处理12小时，分别产生50和110分钟的G1和S延迟。一部分中期细胞（处理4或12小时后分别为12或50%）证明染色体聚集，无法完成胞质分裂，并在下一次分裂中作为四倍体细胞出现。这些四倍体细胞不能存活并产生肉眼可见的集落。它的结论是，这种活力下降是由不可逆的影响纺锤体和/或中心粒组件位于前纺锤体激活标记的中期细胞。
Chinese hamster mitotic cells (95% in mitosis with 85% in metaphase and 10% in anaphase) were shaken loose from monolayer asynchronous cultures and stored at 1°C for up to 28 hours. During this period, the mitotic index did not decrease and the cells remained cytologically normal. However, over a four‐hour period, metaphase cells located within 1.8 minutes of anaphase, 10% of the metaphase population, were able to move into anaphase; this point of 1.8 minutes corresponds in time to that reported for the spindle activation marker. When the cells were warmed to 37°C, they were delayed in entering G1 and S (35 and 70 minutes, respectively, after a 4‐hour treatment). This delay of 70 minutes was maintained for three cell cycles, during which a high degree of synchrony was maintained. Cold treatment for 12 hours produced delays into G1 and S of 50 and 110 minutes, respectively. A fraction of the metaphase cells (12 or 50% after treatments of 4 or 12 hours, respectively) evidenced chromosomal aggregation, were unable to complete cytokinesis, and appeared in the next division as tetraploid cells. These tetraploid cells were unable to survive and produce macroscopic colonies. It is concluded that this decrease in viability is caused by irreversible effects on the spindle and/or centriolar components in metaphase cells located prior to the spindle activation marker.