Effect of Tamarindus indica L. and Manihot esculenta Extracts on Antibiotic-resistant Bacteria
Effect of Tamarindus indica L. and Manihot esculenta Extracts on Antibiotic-resistant Bacteria
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DOI：
10.4103/0974-8490.204648
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发表时间：
2017-04-01
影响因子：
0.7
通讯作者：
Hernandez Macedo, Maria Lucila
中科院分区：
文献类型：
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作者：
Lima, Zenon Machado;da Trindade, Lenilson Santos;Hernandez Macedo, Maria Lucila
背景资料：传统医学中使用的植物的化学成分表现出生物活性化合物，如单宁，黄酮类化合物和生物碱，并成为治疗微生物感染（主要是耐药细菌）的有前途的方法。目的：本研究的目的是评估罗望子（罗望子）和木薯（木薯）的水乙醇叶提取物对铜绿假单胞菌临床分离株和耐甲氧西林金黄色葡萄球菌的抗菌潜力。材料与方法：水乙醇叶提取物的制备和其特征在于通过高效液相色谱/二极管阵列检测器，傅立叶变换红外，1，1-二苯基-2-苦肼，和紫外-可见光的方法。通过微量稀释法在最低抑菌浓度下评价对四种临床相关菌株的抗菌活性。结果：在植物提取物中检测到黄酮类等酚类化合物。T. 500 μ g/mL的籼稻提取物对S. aureus和铜绿假单胞菌;在该浓度下，食用菌仅显示出抗铜绿假单胞菌的活性。结论：我们的研究结果表明，多酚和黄酮类化合物存在于T。indica叶提取物是抗微生物化合物的潜在来源。霸王结果表明，提取物对沙门氏菌有一定的抑菌活性。aureus和铜绿假单胞菌;番茄红素仅对美罗培南耐药的铜绿假单胞菌有效。
Background: The chemical composition of plants used in traditional medicine exhibits biologically active compounds, such as tannins, flavonoids, and alkaloids and becomes a promising approach to treat microbial infections, mainly with drug-resistant bacteria. Objective: The aim of the present study was to evaluate the hydroethanolic leaf extracts of Tamarindus indica (tamarind) and Manihot esculenta (cassava) as antimicrobial potential against Pseudomonas aeruginosa clinical isolated and Methicillin-resistant Staphylococcus aureus. Materials and Methods: Hydroethanolic leaf extracts were prepared and characterized by high-performance liquid chromatography/diode array detection, Fourier transform infrared, 1,1-diphenyl-2-picrylhydrazyl, and ultraviolet-visible methods. The antimicrobial activity against four strains of clinical relevance was evaluated by the microdilution method at minimum inhibitory concentrations. Results: Phenolic compounds such as flavonoids were detected in the plant extracts. T. indica extract at 500 mu g/mL showed antimicrobial activity against S. aureus and P. aeruginosa; however, M. esculenta showed only activity against P. aeruginosa in this concentration. Conclusions: Our results suggested that polyphenols and flavonoids present in T. indica leaf extracts are a potential source of antimicrobial compound. The T. indica extract showed antibacterial activity against S. aureus and P. aeruginosa while M. esculenta had effect only on P. aeruginosa meropenem resistant.