Substitution of drinking water by fructose solution induces hyperinsulinemia and hyperglycemia in hamsters
Substitution of drinking water by fructose solution induces hyperinsulinemia and hyperglycemia in hamsters
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DOI：
10.1590/s1807-59322007000300019
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发表时间：
2007-01-01
期刊：
影响因子：
2.7
通讯作者：
Bouskela, Eliete
中科院分区：
文献类型：
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作者：
Barros, Carlos Magno M. R.;Lessa, Rosane Q.;Bouskela, Eliete
目得：为了测试在仓鼠中获得实用且稳定的高胰岛素血症和高血糖症模型的可能性，用10%或20%果糖溶液代替饮用水，持续2、4或6个月。对照组Ia（n = 51）接受过滤水，Ib组（n = 49）接受10%果糖溶液代替水，Ic组（n=8）接受20%果糖溶液代替水。4个月：IIa组对照（n=8），IIb组10%果糖（n = 7），IIc组20%果糖（FIIc，n = 7）。6个月：IIIa组对照（n = 6），IIIb组10%果糖（n = 6），IIIc组20%果糖（n = 5）。所有组均饲喂相同的实验室饲料。在研究期间，每2周对动物称重一次。在每次实验的最后一天（分别为研究开始后第61、121和181天），对动物进行称重并麻醉以采集血液，以测定禁食至少12小时后的血糖和胰岛素。结果：与对照组相比，饮用10%或20%果糖溶液的动物体重增加显著（P < 0.05）。
PURPOSE: To test the possibility of obtaining a practical and stable model of hyperinsulinemia and hyperglycemia in hamsters, substituting the drinking water by 10% or 20% fructose solutions for a period of 2, 4, or 6 months.METHODS: Male hamsters were divided into 3 main groups, further divided in 3 subgroups: Two months: Group Ia control (n = 51) received filtered water, Group Ib (n = 49) received 10% fructose solution instead of water, Group Ic (n=8) received 20% fructose solution instead of water. Four months: Group IIa control (n=8), Group IIb 10% fructose (n = 7), Group IIc 20% fructose (FIIc, n = 7). Six months: Group IIIa control (n = 6), Group IIIb 10% Fructose (n = 6), Group IIIc 20% Fructose (n = 5). All groups were fed with the same laboratory diet. The animals were weighed every 2 weeks during the study period. On the final day of each experiment (61st, 121st, and 181st day after the beginning of the study, respectively), the animals were weighed and anesthetized for blood collection to determine plasma glucose and insulin after at least a 12-h fast. Ten animals of group la and 10 of group Ib were evaluated to determine changes in macromolecular permeability induced by ischemia/reperfusion as measured in the cheek pouch microcirculation.RESULTS: Compared to controls, the animals that drank the 10% or 20% fructose solution had significantly greater weight gain (P