Microvascular oxygen partial pressure during hyperbaric oxygen in diabetic rat skeletal muscle.
Microvascular oxygen partial pressure during hyperbaric oxygen in diabetic rat skeletal muscle.
复制标题
糖尿病大鼠骨骼肌高压氧过程中的微血管氧分压。
DOI：
10.1152/ajpregu.00380.2015
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Mikiyasu Shirai,
中科院分区：
文献类型：
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作者：
Kohei Yamakoshi;Kazuyoshi Yagishita;Hirotsugu Tsuchimochi;Tadakatsu Inagaki;Mikiyasu Shirai,
高压氧（HBO）是治疗糖尿病患者皮肤和底层肌肉缺血性溃疡的主要治疗方法。这些病变不能有效愈合，部分原因是由于糖尿病引起的心血管功能障碍导致的缺氧微血管O2分压（PmvO 2），这改变了O2输送率（Q ~ o2）和利用率（V ~ o2）之间的动态平衡。我们测试的假设，HBO在糖尿病肌肉会加剧高氧PmvO 2动力学，部分原因是减少或减缓心血管，交感神经和呼吸系统对急性HBO暴露的反应。成年雄性Wistar大鼠随机分为糖尿病组（DIA：链脲佐菌素ip）和健康对照组（对照组）。采用小动物高压舱，以0.2ATA/min的速率将氧（100%O2）加压至3.0大气压，用磷光猝灭法测定HBO期间麻醉大鼠胫骨前肌PmvO 2。腰交感神经活动（LSNA），心率（HR）和呼吸频率（RR）进行电生理学测量。在常压高氧和高压氧下，胫前肌DIA PmvO 2增加快于CONT（平均反应时间，CONT 78 ± 8，DIA 55 ± 8 s，P< 0.05）。随后，PmvO 2在CONT和DIA肌肉中保持在相似的水平上升高，直到常压常氧恢复，其中DIA PmvO 2保持其高氧水平的时间长于CONT。DIA组交感神经系统、心脏和呼吸系统对高压氧的反应较CONT组慢。DIA组的RR（CONT：6 ± 1 s，DIA：29 ± 4 s，P< 0.05）、HR（CONT：16 ± 1 s，DIA：45 ± 5 s，P< 0.05）和LSNA（CONT：140 ± 16 s，DIA：247 ± 34 s，P< 0.05）的平均反应时间均大于CONT组。HBO治疗增加胫骨前肌PmvO 2更迅速，持续时间更长的DIA比CONT，但没有达到更高的水平。而呼吸，心血管，和LSNA反应HBO深刻放缓DIA，只有心血管臂（通过HR）可能有助于肌肉血管功能不全和这些更快的PmvO 2动力学。
Hyperbaric oxygen (HBO) is a major therapeutic treatment for ischemic ulcerations that perforate skin and underlying muscle in diabetic patients. These lesions do not heal effectively, in part, because of the hypoxic microvascular O2partial pressures (PmvO2) resulting from diabetes-induced cardiovascular dysfunction, which alters the dynamic balance between O2delivery (Q̇o2) and utilization (V̇o2) rates. We tested the hypothesis that HBO in diabetic muscle would exacerbate the hyperoxic PmvO2dynamics due, in part, to a reduction or slowing of the cardiovascular, sympathetic nervous, and respiratory system responses to acute HBO exposure. Adult male Wistar rats were divided randomly into diabetic (DIA: streptozotocin ip) and healthy (control) groups. A small animal hyperbaric chamber was pressurized with oxygen (100% O2) to 3.0 atmospheres absolute (ATA) at 0.2 ATA/min. Phosphorescence quenching techniques were used to measure PmvO2in tibialis anterior muscle of anesthetized rats during HBO. Lumbar sympathetic nerve activity (LSNA), heart rate (HR), and respiratory rate (RR) were measured electrophysiologically. During the normobaric hyperoxia and HBO, DIA tibialis anterior PmvO2increased faster (mean response time, CONT 78 ± 8, DIA 55 ± 8 s,P< 0.05) than CONT. Subsequently, PmvO2remained elevated at similar levels in CONT and DIA muscles until normobaric normoxic recovery where the DIA PmvO2retained its hyperoxic level longer than CONT. Sympathetic nervous system and cardiac and respiratory responses to HBO were slower in DIA vs. CONT. Specifically the mean response times for RR (CONT: 6 ± 1 s, DIA: 29 ± 4 s,P< 0.05), HR (CONT: 16 ± 1 s, DIA: 45 ± 5 s,P< 0.05), and LSNA (CONT: 140 ± 16 s, DIA: 247 ± 34 s,P< 0.05) were greater following HBO onset in DIA than CONT. HBO treatment increases tibialis anterior muscle PmvO2more rapidly and for a longer duration in DIA than CONT, but not to a greater level. Whereas respiratory, cardiovascular, and LSNA responses to HBO are profoundly slowed in DIA, only the cardiovascular arm (via HR) may contribute to the muscle vascular incompetence and these faster PmvO2kinetics.