A panel of microRNA signature in serum for colorectal cancer diagnosis.
A panel of microRNA signature in serum for colorectal cancer diagnosis.
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血清中用于诊断结直肠癌的一组 microRNA 特征
DOI：
10.18632/oncotarget.15059
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发表时间：
2017-03-07
期刊：
影响因子：
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通讯作者：
Liu P
中科院分区：
文献类型：
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作者：
Zhu M;Huang Z;Zhu D;Zhou X;Shan X;Qi LW;Wu L;Cheng W;Zhu J;Zhang L;Zhang H;Chen Y;Zhu W;Wang T;Liu P
血清中特异性microRNA（miRNAs）表达异常被认为是结直肠癌（CRC）有前途的诊断生物标志物。在初始筛选阶段，通过基于定量逆转录聚合酶链反应（qRT-PCR）的panel，用3个CRC样本池和1个正常对照（NC）样本池，共选择32个差异表达的miRNA。使用qRT-PCR，在训练（30个CRC对30个NC）和测试阶段（136个CRC对90个NC）中进一步确认所选择的血清miRNA。我们发现CRC患者血清中miR-19 a-3 p、miR-21- 5 p和miR-425- 5 p的水平显著高于NC患者。对于训练、测试和外部验证阶段，三种miRNA组的受试者工作特征（ROC）曲线下面积分别为0.86、0.74和0.87（30个CRC对18个NC）。值得注意的是，在CRC组织中也观察到三种miRNA的表达升高（n = 24）。此外，三种miRNA的表达水平在来自CRC血清样品的外来体中显著升高（n = 10）。总之，我们确定了用于诊断CRC的血清三miRNA组。
Dysregulated expression of specific microRNAs (miRNAs) in serum has been recognised as promising diagnostic biomarkers for colorectal cancer (CRC). In the initial screening phase, a total of 32 differentially expressed miRNAs were selected by quantitative reverse transcription polymerase chain reaction (qRT-PCR) based Exiqon panel with 3 CRC pool samples and 1 normal control (NC) pool. Using qRT-PCR, selected serum miRNAs were further confirmed in training (30 CRC VS. 30 NCs) and testing stages (136 CRC VS. 90 NCs). We identified that serum levels of miR-19a-3p, miR-21-5p and miR-425-5p were significantly higher in patients with CRC than in NCs. The areas under the receiver operating characteristic (ROC) curve of the three-miRNA panel were 0.86, 0.74 and 0.87 for the training, testing and the external validation stages (30 CRC VS. 18 NCs), respectively. Significantly, elevated expression of the three miRNAs was also observed in CRC tissues (n = 24). Furthermore, the expression levels of the three miRNAs were significantly elevated in exosomes from CRC serum samples (n = 10). In conclusion, we identified a serum three-miRNA panel for the diagnosis of CRC.