H-1 NMR spectroscopic imaging of myocardial triglycerides in excised dog hearts subjected to 24 hours of coronary occlusion
H-1 NMR spectroscopic imaging of myocardial triglycerides in excised dog hearts subjected to 24 hours of coronary occlusion
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DOI：
10.1161/01.cir.93.7.1464
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发表时间：
1996-04-01
期刊：
影响因子：
37.8
通讯作者：
Pohost, GM
中科院分区：
文献类型：
--
作者：
StreaterKnowlen, IM;Evanochko, WT;Pohost, GM
背景心肌缺血损伤导致脂肪酸β-氧化抑制和脂肪酸酯化增加以及甘油三酯（TG）积累。这种积聚已被证明发生在梗塞周围血流减少的区域，即危险区域。为了评估是否可以检测缺血24小时后犬心脏中TG积累的程度，我们应用心肌H-1核磁共振（NMR）波谱成像（SI）。方法与结果7只成年杂种狗接受了24小时的左冠状动脉前降支闭塞。死后，切除心脏并确定梗塞的大小和位置。使用飞利浦 1.5-T 临床 NMR 成像/光谱系统，进行二维 (2D) H-1 NMR SI。 TG H-1 NMR 化学位移图像通过数值积分从频域谱重建。具有统计显着性（P
Background Myocardial ischemic insult causes depression of fatty-acid beta-oxidation and increased fatty-acid esterification with triglyceride (TG) accumulation. This accumulation has been demonstrated to occur in the territory with diminished blood flow surrounding an infarct, ie, the region at risk. To evaluate whether the extent of TG accumulation in the canine heart after 24 hours of ischemia could be detected, we applied myocardial H-1 nuclear magnetic resonance (NMR) spectroscopic imaging (SI).Methods and Results Seven adult mongrel dogs underwent 24 hours of left anterior descending coronary artery occlusion. Postmortem, the hearts were excised and the size and location of the infarct were determined. With a Philips 1.5-T clinical NMR imaging/spectroscopic system, two-dimensional (2D) H-1 NMR SI was performed. TG H-1 NMR chemical shift images were reconstructed from the frequency domain spectra by numerical integration. A statistically significant (P