Identification of the immunosuppressive components of canine pulmonary surface active material.
Identification of the immunosuppressive components of canine pulmonary surface active material.
复制标题
犬肺表面活性物质免疫抑制成分的鉴定。
DOI：
10.4049/jimmunol.125.3.1093
复制
发表时间：
1980
影响因子：
4.4
通讯作者：
B. Benson
中科院分区：
文献类型：
--
作者：
M. Ansfield;B. Benson
肺表面活性物质（SAM），从犬肺灌洗液中纯化，是一种富含磷脂的脂蛋白，具有强效的免疫抑制活性。进行实验以鉴定SAM中负责这种免疫抑制作用的那些组分。结果表明，脂质，而不是蛋白质，部分SAM是免疫抑制。两种磷脂，磷脂酰甘油和磷脂酰胆碱，被确定为SAM-脂质部分的主要免疫抑制成分。完整SAM、SAM-脂质、磷脂酰甘油和磷脂酰胆碱可抑制淋巴细胞对促有丝分裂或同种异体刺激的增殖。这些制剂中的每一种都抑制有丝分裂原刺激的淋巴细胞的RNA、蛋白质和DNA合成。抗氧化剂始终未能减少SAM或其脂质成分的免疫抑制特性。SAM及其磷脂成分的免疫抑制作用机制尚不清楚。然而，我们的数据表明，这是不太可能的，因为无论是细胞毒性或人工制品的脂质氧化在体外。
Pulmonary surface active material (SAM), purified from canine lung lavage fluids, is a phospholipid-rich lipoprotein with potent immunosuppressive activity. Experiments were performed to identify those components of SAM that were responsible for this immunosuppressive effect. Results indicated that the lipid, and not the protein, fraction of SAM was immunosuppressive. Two phospholipids, phosphatidylglycerol and phosphatidylcholine, were identified as the predominant immunosuppressive components of the SAM-lipid fraction. lymphocyte proliferation in response to mitogenic or allogeneic stimulation was suppressed by intact SAM, SAM-lipid, phosphatidylglycerol, and phosphatidylcholine. Each of these preparations inhibited RNA, protein and DNA synthesis by mitogen-stimulated lymphocytes. Antioxidants consistently failed to diminish the immunosuppressive properties of SAM or its lipid components. The mechanism of this immunosuppressive action of SAM and its phospholipid components remains undefined. Our data indicate, however, that it is unlikely to be due either to cytotoxicity or to an artifact of lipid oxidation in vitro.