Inhibition of cellular alpha DNA polymerase and herpes simplex virus-induced DNA polymerases by the triphosphate of BW759U
Inhibition of cellular alpha DNA polymerase and herpes simplex virus-induced DNA polymerases by the triphosphate of BW759U
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DOI：
10.1128/aac.25.2.191
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发表时间：
1984-02
影响因子：
4.9
通讯作者：
M. S. St. Clair;W. H. Miller;R. L. Miller;C. U. Lambe;P. Furman
中科院分区：
文献类型：
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作者：
M. S. St. Clair;W. H. Miller;R. L. Miller;C. U. Lambe;P. Furman
阿昔洛韦类似物BW759U(9-[[2-hydroxy-1-(hydroxymethyl)ethoxy]methyl]guanine)的三磷酸形式抑制了几株1型单纯疱疹病毒的聚合酶(EC2.7.7.7)。两个抗三磷酸阿昔洛韦的聚合酶对BW759U-三磷酸的敏感性与野生型病毒诱导的聚合酶一样敏感(Ki=0.05~0.1微米)。细胞αDNA聚合酶的Ki值比所研究的各种单纯疱疹病毒株诱导的DNA聚合酶的Ki值大35到50倍。将感染1型单纯疱疹病毒KOS株的VERO细胞与阿昔洛韦类似物孵育，可形成大量的(9-[[2-hydroxy-1-(hydroxymethyl)ethoxy]methyl]guanine)三磷酸。
The triphosphate form of the acyclovir analog BW759U (9-[[2-hydroxy-1-(hydroxymethyl)ethoxy]methyl]guanine) inhibited the DNA polymerases (EC 2.7.7.7) from several strains of herpes simplex virus type 1. Two acyclovir triphosphate-resistant DNA polymerases were as sensitive to BW759U-triphosphate as were the DNA polymerases induced by wild-type viruses (Ki = 0.05 to 0.1 microM). The Ki value for cellular alpha DNA polymerase was 35- to 50-fold greater than those for the DNA polymerases induced by the various herpes simplex virus strains investigated. Incubation of Vero cells infected by the KOS strain of herpes simplex virus type 1 with the acyclovir analog resulted in the formation of substantial quantities of (9-[[2-hydroxy-1-(hydroxymethyl)ethoxy]methyl]guanine) triphosphate.