Creation of a long-lifespan ciliated epithelial tissue structure using a 3D collagen scaffold.
Creation of a long-lifespan ciliated epithelial tissue structure using a 3D collagen scaffold.
复制标题
使用 3D 胶原支架创建长寿命的纤毛上皮组织结构。
DOI：
10.1016/j.biomaterials.2009.09.098
复制
发表时间：
2010
期刊：
影响因子：
14
通讯作者：
Mao,Hua
中科院分区：
文献类型：
--
作者：
Wang,Yuchi;Wong,LidB;Mao,Hua
我们描述了一种应用于气液界面的3D胶原凝胶支架，将分离的原代气管-支气管纤毛细胞培养成纤毛上皮组织结构(CET)的方法。这种三维胶原凝胶培养系统能够诱导纤毛发生，并持续为纤毛向CET的生长提供支持、维持、发育、分化和繁殖。由该系统开发的CETS类似于天然和活体纤毛上皮细胞的纤毛异着丝运动以及细胞的形态、组织学和生理药理学特征。通过简单明了的维护方案，CETS可以持续数月。CETS的功能纤毛活动的完整性使我们能够在不使用动物的情况下评估许多肺部候选药物的长期效果。
We describe a method of using a 3D collagen gel scaffold applied at the air–liquid interface to culture dissociated primary tracheal-bronchial ciliated cells into a ciliated epithelial tissue structure (CETS). This 3D collagen gel culture system enables the induction of ciliogenesis and continuously provides support, maintenance, development, differentiation and propagation for the growth of cilia into the CETS. The CETS developed by this system resembles the ciliary metachronal motility and morphological, histological and physiopharmacological characteristics of cells found in native and in vivo ciliated epithelia. The CETS can be sustained for months with a straightforward and simple maintenance protocol. The integrity of the functional ciliary activity of this CETS enables the evaluation of long-term effects of many pulmonary drug candidates without using animals.