Myostatin inhibits differentiation of bovine preadipocyte
Myostatin inhibits differentiation of bovine preadipocyte
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DOI：
10.1016/j.domaniend.2005.12.001
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发表时间：
2007-01-01
影响因子：
2.1
通讯作者：
Yano, Hideo
中科院分区：
文献类型：
--
作者：
Hirai, Shizuka;Matsumoto, Haruka;Yano, Hideo
本实验研究了肌肉生长抑制素对牛前脂肪细胞分化的影响。含有前脂肪细胞的间质-血管细胞是从大约30个月龄的日本黑牛的肾周脂肪组织中制备的。融合后用1-甲基-3-异丁基-黄嘌呤、地塞米松、胰岛素和曲格列松诱导分化2天，然后培养6天。在分化诱导期(分化早期)或整个分化期，对细胞进行肌肉抑制素处理。培养结束后，检测细胞的3-磷酸甘油脱氢酶活性、脂肪细胞脂肪积累和脂肪细胞脂肪酸结合蛋白基因的表达等终末分化指标。在整个分化过程中，肌肉抑制素处理严重抑制了所有分化标记物的诱导。分化早期的Myostatin处理也抑制了终末分化标志物的诱导，但在整个分化过程中，抑制Myostatin的剂量是其处理的3倍。肌肉生长抑制素治疗可降低PPAR-γ基因的表达，并干扰CCAAT/增强子结合蛋白(C/EBP)α基因的表达。我们还观察到，在肌肉抑制素存在的情况下，卵泡抑素可以刺激前脂肪细胞的分化。这些结果表明，Myostatin通过抑制PPAR-γ和C/EBPαmRNA的表达来抑制牛前脂肪细胞的分化，而卵泡抑素则抵消了myostatin的抑制作用。(C)2005 Elsevier Inc.保留所有权利。
We investigated the effect of myostatin on the differentiation of bovine preadipocyte. Stromal-vascular cells containing preadipocytes were prepared from perirenal adipose tissue of approximately 30-month-old Japanese Black steers. After confluence, the differentiation was induced by 1-methyl-3-isobutyl-xanthine, dexamethasone, insulin, and troglitasone for 2 days, and then subsequently cultured for 6 days. The cells were treated with myostatin during the induction of differentiation (the early phase of differentiation) or throughout the differentiation period. We measured the terminal differentiation markers such as glycerol-3-phosphate dehydrogenase activity, lipid accumulation, and the expression of adipocyte fatty acid-binding protein mRNA at the end of cultures. The treatment with myostatin throughout the differentiation period severely suppressed the induction of all differentiation markers. The treatment with myostatin in the early phase of differentiation also suppressed the induction of terminal differentiation markers but three-fold higher dose of myostatin was required for the suppression compared with its treatment throughout the differentiation period. Myostatin treatment reduced the expression of peroxisome proliferator-activated receptor (PPAR) gamma mRNA and interfered with the induction of CCAAT/enhancer binding protein (C/EBP) a mRNA. We also observed that follistatin stimulates preadipocyte differentiation in the presence of myostatin. These results suggest that myostatin inhibits bovine preadiopocyte differentiation through suppressing PPAR gamma and C/EBP alpha mRNA expressions and that follistatin counteracts the suppressive effect of myostatin. (c) 2005 Elsevier Inc. All rights reserved.