Decrease of cytotoxic T cells in allergic asthma correlates with total serum immunglobulin E
Decrease of cytotoxic T cells in allergic asthma correlates with total serum immunglobulin E
复制标题
DOI：
10.1111/j.1398-9995.2006.01192.x
复制
发表时间：
2006-11-01
期刊：
影响因子：
12.4
通讯作者：
Virchow, J. C., Jr.
中科院分区：
文献类型：
--
作者：
Bratke, K.;Haupt, F.;Virchow, J. C., Jr.
背景资料：过敏性哮喘与辅助性T细胞2型样细胞因子和T细胞的增加有关，但越来越多的证据表明淋巴细胞介导的细胞毒性机制在哮喘发病机制中的作用。因此，我们研究了过敏性asthma.Methods：颗粒酶A，B，K，和穿孔素在外周血淋巴细胞中的表达，采用流式细胞术的不同淋巴细胞亚群的细胞毒性潜力。可溶性颗粒酶测定血清中使用特定的酶联免疫吸附试验。结果：哮喘患者的颗粒酶和穿孔素阳性的CD 4 T细胞的百分比显着下降，与非过敏性对照。在哮喘患者中，颗粒酶B和穿孔素阳性亚群的CD 8（+）T细胞和自然杀伤T细胞（代表分化程度更高的细胞群）显著减少，而在分化程度较低的颗粒酶K+亚群中未观察到这种情况。此外，血清颗粒酶B浓度在哮喘患者中显著降低，而颗粒酶K浓度无差异。T细胞亚群中颗粒酶A、B与穿孔素表达呈负相关，血清颗粒酶B浓度与血清免疫球蛋白E总量呈负相关。在CD 3-阴性的自然杀伤细胞，颗粒酶或穿孔素的表达与哮喘患者和controls.Conclusion之间没有差异：在过敏性哮喘，细胞毒性T淋巴细胞亚群的分化表型显着下降，这是相关的血清免疫球蛋白E水平。
Background: Allergic asthma has been linked to an increase in T-helper type 2-like cytokines and T cells, but there is growing evidence for a role of lymphocyte-mediated cytotoxic mechanisms in the pathogenesis of asthma. Therefore, we investigated the cytotoxic potential of different lymphocyte subpopulations in patients with allergic asthma.Methods: Granzyme A, B, K, and perforin expression in peripheral blood lymphocytes was analyzed using flow cytometry. Soluble granzymes were measured in serum using specific enzyme-linked immunosorbent assays.Results: Asthmatics had significantly decreased percentages of granzyme and perforin-positive CD4 T cells compared with non-atopic controls. In patients with asthma, the granzyme B and perforin-positive subset of CD8(+) T cells and natural killer T cells, which represent more differentiated cell populations, were significantly reduced, while this was not observed in the less differentiated granzyme K+ subsets. In addition, the serum concentrations of granzyme B were significantly reduced in patients with asthma, while granzyme K concentrations were not different. Interestingly, there was a negative correlation between granzyme A, B and perforin expression in T cell subsets as well as serum granzyme B concentrations and total serum immunglobulin E. In CD3-negative natural killer cells, no differences in granzyme or perforin expression between patients with asthma and controls were detected.Conclusion: In allergic asthma, cytotoxic T lymphocyte subsets of a more differentiated phenotype are significantly decreased and this is correlated to serum immunglobulin E levels.