Bacteria Endosymbiont, Wolbachia, Promotes Parasitism of Parasitoid Wasp Asobara japonica.
Bacteria Endosymbiont, Wolbachia, Promotes Parasitism of Parasitoid Wasp Asobara japonica.
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DOI：
10.1371/journal.pone.0140914
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hayakawa Y
中科院分区：
文献类型：
--
作者：
Furihata S;Hirata M;Matsumoto H;Hayakawa Y
沃尔巴克氏菌是分布最广泛的内共生细菌，它通过控制节肢动物宿主的繁殖来增强自身在宿主种群中的传播。感染沃尔巴克氏菌会导致许多膜翅目的完全孤雌生殖，只产生雌性后代。沃尔巴克氏菌控制生殖的机制已被广泛研究。然而，沃尔巴克氏菌共生对寄主行为特征的影响却鲜有报道。寄生小蜂Asobara Japan onica是研究这一现象的理想昆虫，因为可以获得共生和非共生的品系：最初在日本主岛和西南部岛屿上采集的感染沃尔巴克氏菌的东京(Wolbachia)菌株(TK)和未感染的Iriomote(IR)菌株。我们比较了两个品系的产卵行为，发现TK品系雌性寄生黑腹果蝇幼虫比IR品系更活跃，尤其是在羽化后的前两天。用四环素或利福平去除TK品系黄蜂体内的沃尔巴克氏菌，可将它们的寄生活性降低到IR品系的水平。对两个品系黄蜂的形态和行为分析表明，沃尔巴克氏菌内共生菌不影响寄主雌性生殖道和卵的发育，但确实增强了雌性黄蜂寻找寄主的能力。这些结果表明，Wolbachia内共生菌可能不仅部分通过孤雌生殖促进其在寄主种群中的扩散和持续，而且还可能通过提高寄主雌性的产卵频率来促进它们的扩散和持续。
Wolbachia is the most widespread endosymbiotic bacterium that manipulates reproduction of its arthropod hosts to enhance its own spread throughout host populations. Infection with Wolbachia causes complete parthenogenetic reproduction in many Hymenoptera, producing only female offspring. The mechanism of such reproductive manipulation by Wolbachia has been extensively studied. However, the effects of Wolbachia symbiosis on behavioral traits of the hosts are scarcely investigated. The parasitoid wasp Asobara japonica is an ideal insect to investigate this because symbiotic and aposymbiotic strains are available: Wolbachia-infected Tokyo (TK) and noninfected Iriomote (IR) strains originally collected on the main island and southwest islands of Japan, respectively. We compared the oviposition behaviors of the two strains and found that TK strain females parasitized Drosophila melanogaster larvae more actively than the IR strain, especially during the first two days after eclosion. Removing Wolbachia from the TK strain wasps by treatment with tetracycline or rifampicin decreased their parasitism activity to the level of the IR strain. Morphological and behavioral analyses of both strain wasps showed that Wolbachia endosymbionts do not affect development of the host female reproductive tract and eggs, but do enhance host-searching ability of female wasps. These results suggest the possibility that Wolbachia endosymbionts may promote their diffusion and persistence in the host A. japonica population not only at least partly by parthenogenesis but also by enhancement of oviposition frequency of the host females.