The Establishment of an Optimal Protocol for Contrast-Enhanced Micro-Computed Tomography in the Cloudy Catshark Scyliorhinus torazame
The Establishment of an Optimal Protocol for Contrast-Enhanced Micro-Computed Tomography in the Cloudy Catshark Scyliorhinus torazame
复制标题
云猫鲨 Scylorhinus torazame 增强显微计算机断层扫描最佳协议的建立
DOI：
10.1002/aah.10143
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发表时间：
2021
影响因子：
1.2
通讯作者：
Sasai K
中科院分区：
文献类型：
--
作者：
Ito T;Furuya M;Sasai K
本研究的目的是确定使用微型CT(μ-CT)对云猫的主干后静脉(PCV)、背主动脉(DA)、肝门静脉(HPV)、肾、肝、头动脉(CA)和鳃进行增强扫描的最佳成像方案。此外，我们还检查了体腔器官CECT筛查的可行性。给6条鲨鱼静脉注射不同剂量(10 0、30 0、5 0 0、70 0 mg碘)的碘帕米多2 0 S。S用药后0~600min行胸腰带至盆带CT扫描。检查了颈动脉、鳃和体腔器官的增强CT成像。信号增强值评估显示，在25 S的所有对比剂剂量下，均能很容易地显示大脑中静脉，在稍高的剂量下可见冠状动脉、鳃和多巴胺(冠状动脉和鳃：40 S：200mgI/kg；DA：300 mgI/kg，50 S)。在S延迟150次后，HPV在至少500mgI/kg的剂量下明显存在。肾门脉系统对比剂消失后，300mgI/kg、150 S组肾实质有对比剂作用。肝脏储存了大量的脂质，总体对比度增强较差，最高剂量为700mgI/kg时得到优化。S在700mgI/kg、150mgI/kg、150 的CT增强扫描中，对卵巢、输卵管腺、子宫、睾丸等生殖器官的成像效果最佳。本研究结果不仅可应用于临床实践，还可应用于水族馆中弹性肌群的学术研究和教育。
The purpose of this study was to determine the optimal imaging protocol for contrast‐enhanced computed tomography (CECT) using micro‐CT (μ‐CT) for the posterior cardinal vein (PCV), dorsal aorta (DA), hepatic portal vein (HPV), kidney, liver, cephalic arteries (CAs), and gills of Cloudy CatsharksScyliorhinus torazame. Additionally, we examined the availability of CECT screening for the coelomic organs. Different doses of iopamidol (100, 300, 500, and 700 mg iodine [mgI]/kg) were administered intravenously for 20 s in six sharks. The CT scans from the pectoral girdle to the pelvic girdle were performed at 0–600 s after administration. Contrast‐enhanced CT imaging of the CAs, gills, and coelomic organs was examined. Assessment of the signal enhancement value revealed that the PCV was easily visualized with all contrast doses at 25 s. The CAs, gills, and DA were visible at a slightly higher dose (CAs and gills: 200 mgI/kg at 40 s; DA: 300 mgI/kg at 50 s). The HPV was obvious at a dose of at least 500 mgI/kg after a 150‐s delay. The parenchyma of the kidney had a contrast effect at 300 mgI/kg, 150 s after the contrast effect of the renal portal system disappeared. The liver, which stores a lot of lipids, had poor overall contrast enhancement that was optimized at the highest dose of 700 mgI/kg. Contrast‐enhanced CT screening at 700 mgI/kg and 150 s is likely to obtain the optimal imaging of the reproductive organs, such as the ovary, oviducal gland, uterus, and testis. The present findings can be applied not only to clinical practice but also to academic research and education on elasmobranchs in aquariums.