Characterization of Fruit-Type Sucrose Synthase Gene Promoters Isolated from Tomato and Japanese Pear
Characterization of Fruit-Type Sucrose Synthase Gene Promoters Isolated from Tomato and Japanese Pear
复制标题
从番茄和日本梨中分离的水果型蔗糖合酶基因启动子的表征
DOI：
10.6090/jarq.44.17
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
H. Fukuoka
中科院分区：
文献类型：
--
作者：
A. Ohyama;K. Tanase;Keita Suwabe;M. Kunihisa;T. Nunome;H. Fukuoka
利用反向PCR技术从番茄和日本梨果实型蔗糖合成酶（SS）基因中克隆了5'上游启动子区。番茄SS基因（TOMSSF）的5'非翻译区含有一个约1.6kbp的内含子，而日本梨SS基因（PypSUS 1）的5'非翻译区没有内含子。将每个区域与β-葡糖醛酸糖苷酶（GUS）基因融合，然后将每个构建体（TOMSSF 5 '：：GUS或PypSUS 1 5'：：GUS）用于转化番茄。组织化学分析表明，所有转化子的GUS活性在果实中都有较高的活性。对所有转化体果实中的染色模式的分析显示出维管组织和种皮的特异性染色。在所有转化体中，GUS活性和GUS mRNA水平在发育早期的果实中最高。然而，在PypSU 1 5 '：：GUS系的成熟果实中没有观察到该水平的增加，表明PypSU 1 5'：：GUS番茄的表达模式与日本梨中的PypSU 1的表达模式不同。这可能是由于番茄和日本梨在成熟过程中蔗糖合成机制上的遗传背景差异所致。
We isolated the 5' upstream promoter regions of the fruit-type sucrose synthase (SS) gene from tomato and Japanese pear by inverse PCR. The 5' region of the tomato SS gene (TOMSSF) contained an intron approximately 1.6 kbp long in the 5'untranslated region, whereas the equivalent 5' region in the Japanese pear SS gene (PypSUS1) had no intron. Each region was fused to the β-glucuronidase (GUS) gene and then each construct (TOMSSF 5'::GUS or PypSUS1 5'::GUS) was used to transform tomato. Histochemical analysis of GUS activity of all transformants showed high GUS activity in the fruit. Analysis of the staining pattern in the fruits of all transformants showed staining specific to vascular tissues and testae. The highest level of GUS activity and GUS mRNA was found in fruits at earlier stages of development in all transformants. However, the increase in the level was not observed in the ripening fruits of PypSU1 5'::GUS lines, indicating that the expression patterns of PypSUS1 5'::GUS tomato were different from those of PypSUS1 in Japanese pear. It may ascribe to the differences in genetic background between tomato and Japanese pear that relate to the mechanisms contributing to the sucrose synthesis during ripening.
OHTA,S.：“甘薯块根贮藏蛋白基因启动子的茎优势表达：转基因烟草植物中的 GUS 嵌合基因。”
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
--