Analysis of peptides secreted from cultured mouse brain tissue.
Analysis of peptides secreted from cultured mouse brain tissue.
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DOI：
10.1016/j.bbapap.2013.01.043
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发表时间：
2013-11
影响因子：
3.2
通讯作者：
Fricker, Lloyd D.
中科院分区：
文献类型：
--
作者：
Gelman, Julia S.;Dasgupta, Sayani;Berezniuk, Iryna;Fricker, Lloyd D.
肽代表了细胞-细胞信号分子的主要类别。大多数肽组学研究集中在脑或其他组织中存在的肽。对于在细胞间信号传导中起作用的肽，它必须被分泌。本研究旨在鉴定在含氧缓冲液中培养3-4小时的小鼠脑片分泌的主要肽。大约75%的肽培养切片提取物中确定匹配先前报道的热灭活小鼠脑组织的肽含量，而只有2%匹配未加热的脑组织的肽含量，后者显示了大量的死后变化。如发现与热灭活的小鼠脑提取物，培养的脑切片的提取物代表分泌途径肽以及来自细胞内蛋白质的肽，如存在于细胞质和线粒体中的那些。在培养基中检测到培养切片提取物中检测到的肽的子集。绝大多数分泌肽来自细胞内蛋白而不是分泌途径蛋白。在培养基中检测到肽RVD-加压素，CB 1大麻素受体激动剂，这与RVD-加压素作为非经典神经肽的作用一致。与以前的研究一起，本研究结果表明，小鼠脑片的短期培养是研究肽分泌的合适系统，特别是由细胞内蛋白质产生的肽分泌的非常规途径。
Peptides represent a major class of cell-cell signaling molecules. Most peptidomic studies have focused on peptides present in brain or other tissues. For a peptide to function in intercellular signaling, it must be secreted. The present study was undertaken to identify the major peptides secreted from mouse brain slices that were cultured in oxygenated buffer for 3-4 hours. Approximately 75% of the peptides identified in extracts of cultured slices matched the previously reported peptide content of heat-inactivated mouse brain tissue, whereas only 2% matched the peptide content of unheated brain tissue; the latter showed a large number of postmortem changes. As found with extracts of heat-inactivated mouse brain, the extracts of cultured brain slices represented secretory pathway peptides as well as peptides derived from intracellular proteins such as those present in the cytosol and mitochondria. A subset of the peptides detected in the extracts of the cultured slices was detected in the culture media. The vast majority of secreted peptides arose from intracellular proteins and not secretory pathway proteins. The peptide RVD-hemopressin, a CB1 cannabinoid receptor agonist, was detected in culture media, which is consistent with a role for RVD-hemopressin as a non-classical neuropeptide. Taken together with previous studies, the present results show that short-term culture of mouse brain slices is an appropriate system to study peptide secretion, especially the non-conventional pathway(s) by which peptides produced from intracellular proteins are secreted.
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影响因子：
4.4
作者：
Gelman, Julia S.;Sironi, Juan;Castro, Leandro M.;Ferro, Emer S.;Fricker, Lloyd D.
通讯作者：
Fricker, Lloyd D.
影响因子：
--
作者：
Fricker LD
通讯作者：
Fricker LD
DOI：
10.1073/pnas.0813216106
发表时间：
2009-09-08
影响因子：
11.1
作者：
Biagioli, Marta;Pinto, Milena;Gustincich, Stefano
通讯作者：
Gustincich, Stefano
影响因子：
3.4
作者：
Berti, Denise A.;Russo, Lilian C.;Ferro, Emer S.
通讯作者：
Ferro, Emer S.
影响因子：
5.3
作者：
Fricker, LD;McKinzie, AA;Douglass, J
通讯作者：
Douglass, J