Large-scale patterning of living colloids for dynamic studies of neutrophil-microbe interactions.
Large-scale patterning of living colloids for dynamic studies of neutrophil-microbe interactions.
复制标题
生存胶体的大规模模式，用于中性粒细胞 - 微叶相互作用的动态研究。
DOI：
10.1039/c8lc00228b
复制
发表时间：
2018-05-29
期刊：
影响因子：
6.1
通讯作者：
Irimia D
中科院分区：
文献类型：
--
作者：
Kim JJ;Reátegui E;Hopke A;Jalali F;Roushan M;Doyle PS;Irimia D
中性粒细胞是第一个对微生物做出反应并限制其入侵身体的白色血细胞。然而，微生物生长成菌落通常会挑战中性粒细胞容纳它们的能力。为了研究嗜中性粒细胞和微生物菌落之间的相互作用，我们设计了一种用于将微生物排列成受控大小的簇（即活胶体）的测定法。活胶体中的模式化微生物被机械地捕获在威尔斯孔内，并且完全可接近中性粒细胞。使用该检测方法，我们研究了人类中性粒细胞与白色念珠菌和金黄色葡萄球菌，两种常见的人类病原体之间的相互作用。我们还探讨了C.白色念珠菌胶体到卡泊芬净，一种常见的抗真菌药物。我们提出了一种用于微生物簇的大规模图案化的方法，这使得能够研究微生物群落和免疫细胞之间的相互作用。
Neutrophils are the first white blood cells to respond to microbes and to limit their invasion of the body. However, the growth of the microbes into colonies often challenges the neutrophils ability to contain them. To study the interactions between neutrophils and microbial colonies, we designed an assay for arranging microbes in clusters of controlled size (i.e. living colloids). The patterned microbes in the living colloid are mechanically trapped inside the wells and fully accessible to neutrophils. Using the assay, we studied the interactions between human neutrophils and Candida albicans and Staphylococcus aureus, two common human pathogens. We also probed the susceptibility of C. albicans colloids to caspofungin, a common antifungal drug. We present a method for large-scale patterning of microorganism clusters, which enables the study of interaction between microbial communities and immune cells.