Inter-organ signaling in plants: regulation of ATP sulfurylase and sulfate transporter genes expression in roots mediated by phloem-translocated compound
Inter-organ signaling in plants: regulation of ATP sulfurylase and sulfate transporter genes expression in roots mediated by phloem-translocated compound
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DOI：
10.1046/j.1365-313x.1999.00416.x
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发表时间：
1999-04-01
期刊：
影响因子：
7.2
通讯作者：
Touraine, B
中科院分区：
文献类型：
--
作者：
Lappartient, AG;Vidmar, JJ;Touraine, B
缺硫处理增强了拟南芥和甘蓝型油菜根系的硫酸盐吸收和ATP硫酸化酶活性，而再供应SO 42-则降低了根系的硫酸盐吸收和ATP硫酸化酶活性。类似的反应发生在分根实验中，只有一部分的根系被剥夺S，这表明该调节涉及器官间的信号传导。韧皮部转移的谷胱甘肽（GSH）被认为是调节根中SO 42-吸收速率和ATP硫酸化酶活性的可能的转导分子。GSH的调节作用得到证实的发现，ATP硫酸化酶活性被抑制，通过提供半胱氨酸，除了在存在的丁硫氨酸亚砜亚胺，GSH合成的抑制剂。在直接和远程（分根）曝光，蛋白质水平检测到的抗体对拟南芥APS 3 ATP硫酸化酶增加的根。thaliana和B.恢复SO 42-后，S饥饿时，油菜的抗氧化能力下降，补充GSH后，抗氧化能力下降。RNA印迹分析显示，直接和远程GSH处理降低了编码ATP硫酸化酶的APS 1的转录水平。AST 68（编码SO 42-转运蛋白的基因）的丰度也受到硫状态改变的影响。这份报告提出了第一个证据的监管根基因参与营养物质的获取和同化的信号，是从地上部转移到根。
Sulfate uptake and ATP sulfurylase activity in the roots of Arabidopsis thaliana and Brassica napus were enhanced by S deprivation and reduced following resupply of SO42-. Similar responses occurred in split-root experiments where only a portion of the root system was S deprived, suggesting that the regulation involves inter-organ signaling. Phloem-translocated glutathione (GSH) was identified as the likely transducing molecule responsible for regulating SO42- uptake rate and ATP sulfurylase activity in roots. The regulatory role of GSH was confirmed by the finding that ATP sulfurylase activity was inhibited by supplying Cys except in the presence of buthionine sulfoximine, an inhibitor of GSH synthesis. In direct and remote (split-root) exposures, levels of protein detected by antibodies against the Arabidopsis APS3 ATP sulfurylase increased in the roots of A. thaliana and B. napus during S starvation, decreased after SO42- restoration, and declined after feeding GSH. RNA blot analysis revealed that the transcript level of APS1, which codes for ATP sulfurylase, was reduced by direct and remote GSH treatments. The abundance of AST68 (a gene encoding an SO42- transporter) was similarly affected by altered sulfur status. This report presents the first evidence for the regulation of root genes involved in nutrient acquisition and assimilation by a signal that is translocated from shoot to root.