THE DIAGNOSTIC-SIGNIFICANCE OF THE DIRECT ANTIGLOBULIN-TEST (DAT) IN ANEMIC DOGS
THE DIAGNOSTIC-SIGNIFICANCE OF THE DIRECT ANTIGLOBULIN-TEST (DAT) IN ANEMIC DOGS
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DOI：
10.1016/0165-2427(79)90007-2
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发表时间：
1979-01-01
影响因子：
1.8
通讯作者：
SLAPPENDEL, RJ
中科院分区：
文献类型：
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作者：
SLAPPENDEL, RJ
The direct antiglobulin test (DAT) was positive in 134 (36.1%) of 371 anemic dogs with internal diseases.识别出四种主要反应类型：15 例 (11.2%) 中单独出现 IgG [抗狗 IgG 抗体]，41 例 (30.6%) 中出现 IgG + C'' [抗狗补体成分 3b 抗体]，74 例 (55.2%) 中出现单独 C''，2 例 (1.5%) 中出现 IgM [抗人 IgM 抗体] + C''。极少数情况下，IgM 和/或 IgA 反应与强烈的 IgG + C'' 反应同时发生。 In 2 (1.5%) DAT-positive dogs the type of reaction was unclear. 84只DAT阳性犬表现出一种或多种溶血症状，如血红蛋白血症、间接型高胆红素血症、红细胞渗透脆性增加和粪便尿胆原排泄增加。其中包括 15 只狗中的 10 只具有 IgG 型 DAT、41 只狗中的 36 只具有 IgG + C” 型 DAT、74 只狗中的 36 只具有 C” 型 DAT 以及 2 只狗中的 2 只具有 IgM + C” 型 DAT。大多数出现 IgG + C'' 型反应的狗有严重溶血，而 56 例中只有 26 例发现了原发或相关疾病。 IgG 型不完全温抗体与汇集的供体细胞发生反应，在 3 只具有 IgG 型 DAT 的狗的红细胞洗脱物中得到证实，并且在 7 只具有 IgG + C'' 型反应的狗中的 6 只中得到证实。显然，有 IgG 或 IgG + C'' 反应的狗通常患有自身免疫性溶血性贫血。在患有 C'' 型 DAT 的狗中，溶血迹象通常很轻微或不存在。症状几乎总是表明存在某种原发性疾病。诊断主要包括感染、炎症和肿瘤（特别是骨髓和淋巴增殖性）疾病。在 74 只患有 C'' 型 DAT 的狗中，只有 7 只（9.5%）除了（短暂性超急性）溶血性贫血外没有做出其他诊断。患有 C'' 型 DAT 的狗的血清和红细胞洗脱液中的抗体检测结果始终为阴性。 In 1 dog with hemolytic anemia and C'' + IgM type DAT, there was a high titer IgM cold agglutinins in the serum and in heat eluates.抗 IgG 抗血清的 DAT 阳性是自身免疫性溶血性贫血的强烈指征，但单独 C'' 型反应是犬内科疾病中相当常见的现象，很少与严重溶血相关。
The direct antiglobulin test (DAT) was positive in 134 (36.1%) of 371 anemic dogs with internal diseases. Four principal types of reaction were recognized: IgG [anti-dog IgG antibodies] alone in 15 (11.2%), IgG + C'' [anti-dog complement component 3b antibodies] in 41 (30.6%), C'' alone in 74 (55.2%) and IgM [anti-human IgM antibodies] + C'' in 2 (1.5%). Rarely, IgM and/or IgA reactions occurred in association with strong IgG + C'' reactions. In 2 (1.5%) DAT-positive dogs the type of reaction was unclear. One or more hemolysis symptoms such as hemoglobinemia, indirect type hyperbilirubinemia, increased red cell osmotic fragility and increased fecal urobilinogen excretion were demonstrated in 84 DAT-positive dogs. These consisted of 10 of 15 dogs with IgG type DAT, 36 of 41 dogs with IgG + C'' type DAT, 36 of 74 dogs with C'' type DAT and 2 of 2 dogs with IgM + C'' type DAT. Most dogs with IgG + C'' type reactions had severe hemolysis, whereas primary or associated diseases were recognized in only 26 of 56 cases. IgG type incomplete warm antibody, reacting with pooled donor cells, was demonstrated in red cell eluates in each of 3 dogs with IgG type DAT and in 6 of 7 dogs with IgG + C'' type reactions. Apparently dogs with IgG or IgG + C'' reactions usually have autoimmune hemolytic anemia. In dogs with C'' type DAT, indications of hemolysis were frequently minimal or absent. Symptoms almost always indicated some primary disorder. Diagnoses mainly included infections, inflammatory and neoplastic (especially myelo- and lymphoproliferative) diseases. In only 7 (9.5%) of 74 dogs with C'' type DAT no diagnosis other than (transient peracute) hemolytic anemia was made. The tests results for antibodies in the serum and red cell eluates were always negative in dogs with C'' type DAT. In 1 dog with hemolytic anemia and C'' + IgM type DAT, there was a high titer IgM cold agglutinins in the serum and in heat eluates. A positive DAT with anti-IgG antiserum is a strong indication of autoimmune hemolytic anemia, but that a reaction of the C'' alone type is a rather common phenomenon in canine internal diseases which is seldom associated with serious hemolysis.