Screening of Differentiation-Specific Molecular Biomarkers for Colon Cancer
Screening of Differentiation-Specific Molecular Biomarkers for Colon Cancer
复制标题
结肠癌分化特异性分子生物标志物的筛选
DOI：
10.1159/000489660
复制
发表时间：
2018-01-01
影响因子：
--
通讯作者：
Ding, Yanqing
中科院分区：
文献类型：
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作者：
Qi, Lu;Ding, Yanqing
背景/目标：由于目前尚缺乏有效的分子标志物来评价结肠癌的分化程度，筛选有效的分子标志物对结肠癌的诊断和治疗具有重要意义。本研究是利用结肠组织特异性基因筛选与结肠分化相关的分子标记的研究。研究方法：本研究比较不同分化程度结肠癌的表达谱，筛选与分化降低相关的下调基因。IL 22 RA 1基因是由获得的基因与结肠组织特异性基因的交叉衍生的。采用DriverDB和The Human Protein Atlas分析IL 22 RA 1在各种组织细胞中的表达水平，采用Kaplan-Meier法分析IL 22 RA 1与结肠癌患者生存期的相关性，并采用ROC曲线分析IL 22 RA 1诊断分化型结肠癌的特异性和敏感性。结果如下：我们发现IL 22 RA 1基因在高分化、中分化、低分化和未分化结肠癌组织中表达逐渐下调。IL 22 RA 1在结肠组织和结肠癌组织中的RNA和蛋白水平均高于其他正常组织和癌组织。比较IL 22 RA 1在不同癌细胞中的表达发现，IL 22 RA 1在CACO-2结肠癌细胞中的表达显著高于其他癌细胞。生存分析显示IL 22 RA 1基因表达与结肠癌患者的总生存率呈正相关（P= 0.0224）。ROC曲线分析显示IL 22 RA 1表达对II期结肠癌具有良好的特异性和敏感性。结论：IL 22 RA 1可作为结肠癌分化的特异性分子标志物。(C)2018作者（S）由S发布。Karger AG，巴塞尔
Background/Aims: Owing to the lack of effective molecular markers to evaluate colon cancer differentiation grade, screening of effective molecular markers for the diagnosis and treatment of colon cancer is of great significance. This study is a screening study for molecular markers related to the differentiation of colon using the tissue-specific genes of colon. Methods: This study compared the expression profiles of colon cancer at various differentiation grades and screened the down-regulated genes associated with decreased differentiation. IL22RA1 gene was derived from the intersection of obtained gene and colon tissue-specific genes. We used DriverDB and The Human Protein Atlas to analyze the expression level of IL22RA1 in various tissue cells, also used Kaplan-Meier method to analyze the correlation between IL22RA1 and the survival of colon cancer patients, and then used the ROC curve to analyze the specificity and sensitivity of IL22RA1 diagnosis of differentiated colon cancer. Results: We found that IL22RA1 gene expression was progressively down-regulated in high-differentiated, moderatedifferentiated, low-differentiated, and undifferentiated colon cancer tissues. Both RNA and protein levels of IL22RA1 were higher in colon tissues and colon cancer tissues than in other normal and cancer tissues. Comparison of IL22RA1 expression in different cancer cells found that IL22RA1 expression was significantly higher in CACO-2 colon cancer cells than in other cancer cells. Survival analysis showed that IL22RA1 gene expression was positively correlated with the overall survival rate of colon cancer patients (P= 0.0224). ROC curve analysis revealed that IL22RA1 expression had good specificity and sensitivity to stage II colon cancer. Conclusion: These findings suggest that IL22RA1 serves as a specific molecular marker for the differentiation of colon cancer. (C) 2018 The Author(s) Published by S. Karger AG, Basel