Electrospun fibers immobilized with bone forming peptide-1 derived from BMP7 for guided bone regeneration
Electrospun fibers immobilized with bone forming peptide-1 derived from BMP7 for guided bone regeneration
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DOI：
10.1016/j.biomaterials.2013.03.051
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发表时间：
2013-07-01
期刊：
影响因子：
14
通讯作者：
Shin, Heungsoo
中科院分区：
文献类型：
--
作者：
Lee, Young Jun;Lee, Ji-Hye;Shin, Heungsoo
开发具有适当孔隙率和生物活性的理想屏障膜对于引导骨科和颅颌面外科新骨形成至关重要。在这项研究中，我们开发了生物活性的电纺纤维的基础上聚（乳酸-共-乙醇酸）（PLGA）的固定骨形成肽1（BFP 1）来自骨形态发生蛋白7（BMP 7）的未成熟区域。我们利用聚多巴胺化学固定BFP 1;聚多巴胺（PD）的电纺PLGA纤维，其上的BFPI随后在弱碱性条件下固定化。BFP 1的固定化通过表征表面化学组成来验证，并通过荧光胺测定来定量测量。BPF 1在静电纺丝纤维上的固定支持了I型胶原的紧密分布和人间充质干细胞（hMSCs）的铺展。SEM显微照片显示球状矿物质增生的聚集，ALP活性和钙沉积显着增加，当hMSCs在固定BFPI的纤维上培养14天时：然后我们将制备的纤维植入小鼠颅骨缺损，并在2个月后分析骨形成。来自代表性X射线图像的骨生长半定量显示，仅缺陷组的骨面积约为20%，而植入PLGA纤维的组显示出显著改善，分别为44.27 +/- 7.37%和57.59 +/- 15.24%。基于这些结果，我们的方法可能是一个有前途的工具，以开发临床适用的生物活性膜引导骨再生。(C)2013爱思唯尔有限公司保留所有权利。
The development of ideal barrier membranes with appropriate porosity and bioactivity is essential for the guidance of new bone formation in orthopedic and craniomaxillofacial surgery. In this study, we developed bioactive electrospun fibers based on poly (lactide-co-glycolic acid) (PLGA) by immobilizing bone-forming peptide 1 (BFP1) derived from the immature region of bone morphogenetic protein 7 (BMP7). We exploited polydopamine chemistry for the immobilization of BFP1; polydopamine (PD) was coated on the electrospun PLGA fibers, on which BFPI was subsequently immobilized under weakly basic conditions. The immobilization of BFP1 was verified by characterizing the surface chemical composition and quantitatively measured by fluorescamine assay. The immobilization of BPF1 on the electrospun fibers supported the compact distribution of collagen I and the spreading of human mesenchymal stem cells (hMSCs). SEM micrographs demonstrated the aggregation of globular mineral accretions, with significant increases in ALP activity and calcium deposition when hMSCs were cultured on fibers immobilized with BFPI for 14 days: We then implanted the prepared fibers onto mouse calvarial defects and analyzed bone formation after 2 months. Semi-quantification of bone growth from representative X-ray images showed that the bone area was approximately 20% in the defect-only group, while the group implanted with PLGA fibers showed significant improvements of 44.27 +/- 7.37% and 57.59 +/- 15.24% in the groups implanted with PD-coated PLGA and with BFP1-coated PLGA, respectively. Based on these results, our approach may be a promising tool to develop clinically-applicable bioactive membranes for guided bone regeneration. (C) 2013 Elsevier Ltd. All rights reserved.