In vitro ovarian tumor growth and treatment response dynamics visualized with time-lapse OCT imaging
In vitro ovarian tumor growth and treatment response dynamics visualized with time-lapse OCT imaging
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DOI：
10.1364/oe.17.008892
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发表时间：
2009-05-25
期刊：
影响因子：
3.8
通讯作者：
de Boer, Johannes F.
中科院分区：
文献类型：
--
作者：
Evans, Conor L.;Rizvi, Imran;de Boer, Johannes F.
转移性卵巢癌的体外三维模型已用于再现人类疾病。然而，这些球形肿瘤培养物可以生长超过1mm的直径，这在没有合适的成像技术的情况下难以可视化。光学相干层析成像（OCT）是一种理想的非微扰可视化这些复杂系统的实时成像方法。OCT能够在结节和细胞水平上详细观察模型，揭示以前未观察到的生长动力学。能够自动多维采集的延时OCT系统的开发进一步提供了对卵巢癌生长和化疗反应的见解。(c)2009年美国光学学会
In vitro three-dimensional models for metastatic ovarian cancer have been useful for recapitulating the human disease. These spheroidal tumor cultures, however, can grow in excess of 1 mm in diameter, which are difficult to visualize without suitable imaging technology. Optical coherence tomography (OCT) is an ideal live imaging method for non-perturbatively visualizing these complex systems. OCT enabled detailed observations of the model at both nodular and cellular levels, revealing growth dynamics not previously observed. The development of a time-lapse OCT system, capable of automated, multidimensional acquisition, further provided insights into the growth and chemotherapeutic response of ovarian cancer. (c) 2009 Optical Society of America