Co-delivery of paclitaxel and doxorubicin using polypeptide-engineered nanogels for combination therapy of tumor
Co-delivery of paclitaxel and doxorubicin using polypeptide-engineered nanogels for combination therapy of tumor
复制标题
使用多肽工程纳米凝胶共同递送紫杉醇和阿霉素用于肿瘤联合治疗
DOI：
10.1088/1361-6528/ac46b4
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发表时间：
2022
期刊：
影响因子：
3.5
通讯作者：
Bo Liu
中科院分区：
文献类型：
--
作者：
Jie Yang;Rui-Mei Jin;Shen-Yan Wang;Xiao-Ting Xie;Wei Hu;Hong-Feng Tang;Bo Liu
抽象的。将化疗剂装载到纳米颗粒中已被证明是癌症治疗的有效策略。然而，同时将不同功能药物输送到肿瘤部位进行化疗仍然具有挑战性。在本研究中，设计并制备了由工程卷曲螺旋多肽PC10A形成的纳米凝胶作为载体，通过超声波处理和静电吸附共同递送紫杉醇（PTX）和阿霉素（DOX）。 PC10A/PTX/DOX纳米凝胶中PTX和DOX的载药量和包封率分别为5.98 wt%、70 wt%和8.55 wt%、83 wt%。由于多肽PC10A无毒且可生物降解，PC10A/PTX/DOX纳米凝胶表现出良好的生物相容性。体外和体内抗肿瘤实验表明，与游离PTX/DOX相比，PC10A/PTX/DOX纳米凝胶对肿瘤具有明显的协同治疗作用，且副作用较低。因此，PC10A/PTX/DOX纳米凝胶有望为不同功能药物的联合治疗提供新策略。
Abstract. Loading of chemotherapeutic agents into nanoparticles has been demonstrated to be an effective strategy for cancer therapy. However, simultaneous delivery of different functional drugs to tumor sites for chemotherapy still remains challenging. In this study, nanogels formed by an engineered coiled-coil polypeptide PC10A were designed and prepared as a carrier for co-delivery of paclitaxel (PTX) and doxorubicin (DOX) through ultrasonic treatment and electrostatic adsorption. The drug loading content and encapsulation efficiency of PTX and DOX in the PC10A/PTX/DOX nanogels were 5.98 wt%, 70 wt%, and 8.55 wt%, 83 wt%, respectively. Because the polypeptide PC10A was non-toxic and biodegradable, the PC10A/PTX/DOX nanogels exhibited good biocompatibility. The in vitro and in vivo antitumor experiments showed that the PC10A/PTX/DOX nanogels possessed obviously synergistic therapy effect of tumors and lower side effects compared with free PTX/DOX. Therefore, the PC10A/PTX/DOX nanogels are promising to provide a new strategy for combination therapy of different functional drugs.