Effect of lipid composition on activity of liposome-entrapped ampicillin against intracellular Listeria monocytogenes
Effect of lipid composition on activity of liposome-entrapped ampicillin against intracellular Listeria monocytogenes
复制标题
脂质成分对脂质体包埋的氨苄青霉素抗胞内单核细胞增生李斯特菌活性的影响
DOI：
10.1128/aac.32.10.1560
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发表时间：
1988
影响因子：
4.9
通讯作者：
F. Roerdink
中科院分区：
文献类型：
--
作者：
I. Bakker;A. F. Lokerse;F. Roerdink
采用感染单核细胞增生李斯特菌的小鼠腹腔巨噬细胞，研究脂质组成对氨苄青霉素脂质体细胞内抗菌活性的影响。使用了两种类型的脂质体，一种是由胆固醇-磷脂酰胆碱-磷脂酰丝氨酸（5：4：1）组成的流体类型，一种是由胆固醇-二硬脂酰磷脂酰胆碱-二棕榈酰磷脂酰甘油（10：10：1）组成的固体类型。虽然两种类型的脂质体的细胞摄取是相似的，但它们在细胞内降解速率方面不同。发现固体脂质体的相对缓慢的降解与包封的氨苄青霉素的延迟的细胞内释放之间存在相关性，这反映在L.单核细胞增多症
The effect of lipid composition on the intracellular antibacterial activity of ampicillin-containing liposomes was studied in vitro by using mouse peritoneal macrophages infected with Listeria monocytogenes. Two types of liposomes, a fluid type, consisting of cholesterol-phosphatidylcholine-phosphatidylserine (5:4:1), and a solid type, consisting of cholesterol-distearoylphosphatidylcholine-dipalmitoylphosphatidylglyc ero l (10:10:1), were used. Although the cellular uptake of both types of liposomes was similar, they differed with respect to the rate of intracellular degradation. A correlation was found between the relatively slow degradation of the solid liposomes and a delayed intracellular release of the encapsulated ampicillin, as reflected in absent or delayed intracellular killing of L. monocytogenes.