Metabolomic approaches reveal that phosphatidic and phosphatidyl glycerol phospholipids are major discriminatory non-polar metabolites in responses by Brachypodium distachyon to challenge by Magnaporthe grisea
Metabolomic approaches reveal that phosphatidic and phosphatidyl glycerol phospholipids are major discriminatory non-polar metabolites in responses by Brachypodium distachyon to challenge by Magnaporthe grisea
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DOI：
10.1111/j.1365-313x.2006.02692.x
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发表时间：
2006-05-01
期刊：
影响因子：
7.2
通讯作者：
Mur, LAJ
中科院分区：
文献类型：
--
作者：
Allwood, JW;Ellis, DI;Mur, LAJ
代谢组学方法被用来阐明一些关键代谢物的变化发生在相互作用的稻瘟病菌-稻瘟病的原因-与替代主机，二穗短柄草。傅里叶变换红外光谱（FT-IR）提供了一个高通量的代谢指纹M。grisea与B相互作用。二穗草种质ABR 1（感病）和ABR 5（抗病）。主成分判别函数分析（PC-DFA）允许发展中的疾病症状和主机之间的区别。对齐投影的“测试集”的“训练集”的数据表明，我们的实验方法产生了高度可重复的数据。PC-DFA负荷图的检查表明，脂肪酸是区分ABR 1和ABR 5对M的反应的一个化学组。稻瘟病菌为了鉴定这些，M.稻瘟菌攻击的B。直接注入电喷雾电离质谱仪（ESI-MS）。PC-DFA表明M.稻瘟病菌攻击的ABR 1和ABR 5与健康材料不同地聚在一起。减法光谱和PC-DFA加载图显示了每个相互作用之间的歧视性分析物（m/z），随后通过ESI-MS-MS将7种代谢物鉴定为磷脂（PL）。磷脂酰甘油（PG）PL在耐药和敏感反应期间均受到抑制。相比之下，不同的磷脂酸PL增加或减少在阻力或疾病发展过程中。这表明在每个相互作用过程中膜脂质的大量和差异PL处理可能与防御机制的阐述/抑制或疾病症状的发展有关。
Metabolomic approaches were used to elucidate some key metabolite changes occurring during interactions of Magnaporthe grisea- the cause of rice blast disease - with an alternate host, Brachypodium distachyon. Fourier-transform infrared (FT-IR) spectroscopy provided a high-throughput metabolic fingerprint of M. grisea interacting with the B. distachyon accessions ABR1 (susceptible) and ABR5 (resistant). Principal component discriminant function analysis (PC-DFA) allowed the differentiation between developing disease symptoms and host resistance. Alignment of projected 'test-set' on to 'training-set' data indicated that our experimental approach produced highly reproducible data. Examination of PC-DFA loading plots indicated that fatty acids were one chemical group that discriminated between responses by ABR1 and ABR5 to M. grisea. To identify these, non-polar extracts of M. grisea- challenged B. distachyon were directly infused into an electrospray ionization mass spectrometer (ESI-MS). PC-DFA indicated that M. grisea- challenged ABR1 and ABR5 were differentially clustered away from healthy material. Subtraction spectra and PC-DFA loadings plots revealed discriminatory analytes (m/z) between each interaction and seven metabolites were subsequently identified as phospholipids (PLs) by ESI-MS-MS. Phosphatidyl glycerol (PG) PLs were suppressed during both resistant and susceptible responses. By contrast, different phosphatidic acid PLs either increased or were reduced during resistance or during disease development. This suggests considerable and differential PL processing of membrane lipids during each interaction which may be associated with the elaboration/suppression of defence mechanisms or developing disease symptoms.