SiteFinding-PCR: a simple and efficient PCR method for chromosome walking.
SiteFinding-PCR: a simple and efficient PCR method for chromosome walking.
复制标题
DOI：
10.1093/nar/gni124
复制
发表时间：
2005-08-02
影响因子：
14.9
通讯作者：
An C
中科院分区：
文献类型：
--
作者：
Tan G;Gao Y;Shi M;Zhang X;He S;Chen Z;An C
In this paper, we present a novel PCR method, termed SiteFinding-PCR, for gene or chromosome walking. The PCR was primed by a SiteFinder at a low temperature, and then the target molecules were amplified exponentially with gene-specific and SiteFinder primers, and screened out by another gene-specific primer and a vector primer. However, non-target molecules could not be amplified exponentially owing to the suppression effect of stem–loop structure and could not be screened out. This simple method proved to be efficient, reliable, inexpensive and time-saving, and may be suitable for the molecules for which gene-specific primers are available. More importantly, large DNA fragments can be obtained easily using this method. To demonstrate the feasibility and efficiency of SiteFinding-PCR, we employed this method to do chromosome walking and obtained 16 positive results from 17 samples.
登录
查看更多内容
影响因子：
46.9
作者：
Dai, SM;Chen, HH;Flanagan, SD
通讯作者：
Flanagan, SD
影响因子：
7.2
作者：
CHANG, SS;PARK, SK;NAM, HG
通讯作者：
NAM, HG
DOI：
10.1007/s004380051201
发表时间：
2000-04-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
Terauchi, R;Kahl, G
通讯作者：
Kahl, G
影响因子：
14.9
作者：
Yan, YX;An, CC;Chen, ZL
通讯作者：
Chen, ZL
影响因子：
2.6
作者：
Keim, M;Williams, RSB;Harwood, AJ
通讯作者：
Harwood, AJ