Stability of Hydrophilic Magnetic Nanoparticles Under Biologically Relevant Conditions
Stability of Hydrophilic Magnetic Nanoparticles Under Biologically Relevant Conditions
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DOI：
10.1166/jnn.2008.343
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发表时间：
2008-12-01
影响因子：
--
通讯作者：
Gu, N.
中科院分区：
文献类型：
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作者：
Chen, Z. P.;Zhang, Y.;Gu, N.
将亲水性2，3-二巯基丁二酸（HOOC-CH（SH）-CH（SH）-COOH，DMSA）包覆的单分散磁性纳米粒子（Fe 3 O 4）分别分散在水、含10%（v/v）胎牛血清的RPMI-1640、RPMI-1640、PBS和MES中，以研究它们在生物相关条件下的稳定性。光子相关光谱（PCS）结果表明，DMSA-Fe 3 O 4纳米粒子在生物条件下以聚集体的形式存在。UV-vis、MRI和AFM结果表明，分散在RPMI-1640、PBS和MES中的DMSA-Fe 3 O 4纳米粒子稳定性较差，而分散在含胎牛血清的RPMI-1640中的DMSA-Fe 3 O 4纳米粒子稳定性较好，Zeta电位和IR结果证实了这是由于它们从胎牛血清中吸附所致。此外，体外细胞实验表明，胎牛血清吸附的DMSA-Fe 3 O 4纳米粒比未吸附的纳米粒具有更高的细胞内摄取，表明胎牛血清在纳米粒的细胞内摄取中发挥了重要作用。
Hydrophilic 2,3-dimercaptosuccinnic acid (HOOC-CH(SH)-CH(SH)-COOH, DMSA) coated mono-disperse magnetic nanoparticles (Fe3O4) were dispersed in water, RPMI-1640 with 10% (v/v) fetal calf serum, RPMI-1640, PBS and MES, respectively, to investigate their stability under biologically relevant conditions. The Photon Correlation Spectroscopy (PCS) results showed that DMSA-Fe3O4 nanoparticles existed as aggregate under biological conditions. UV-vis, MRI and AFM results indicated that DMSA-Fe3O4 nanoparticles dispersed in RPMI-1640, PBS and MES presented poor stability, whereas those dispersed in RPMI-1640 with fetal calf serum exhibited excellent stability, which was due to their adsorption from fetal calf serum, as confirmed by zeta potential and IR results. Additionally, in vitro cell experiments showed that DMSA-Fe3O4 nanoparticles with adsorption from fetal calf serum had higher intracellular uptake than those without adsorption from serum, indicating that fetal calf serum could play a great role in intracellular uptake of nanoparticles.