A rat model of chronic subdural hematoma: Insight into mechanisms of revascularization and inflammation
A rat model of chronic subdural hematoma: Insight into mechanisms of revascularization and inflammation
复制标题
慢性硬膜下血肿大鼠模型：深入了解血运重建和炎症机制
DOI：
10.1016/j.brainres.2015.08.017
复制
发表时间：
2015-11-02
期刊：
影响因子：
2.9
通讯作者：
Jiang, Rongcai
中科院分区：
文献类型：
--
作者：
Quan, Wei;Zhang, Zhifei;Jiang, Rongcai
慢性硬膜下血肿（CSDH）是老年人群中常见的神经系统疾病，严重影响生活和工作质量。研究试图确定CSDH的危险因素和病理生理机制，使用模型在许多哺乳动物物种。迄今为止，这些动物模型仅能够再现有限持续时间的血肿，这不能准确反映CSDH的慢性状态。为了解决这些挑战中的一些，我们使用连续两次自体血注射改良了CSDH大鼠模型，导致血肿超过三周。我们观察到与CSDH的发展和恢复相关的炎症和血管生成变化。在本研究中，在小动物模型中制备CSDH的技术的成功率为78.13%。血肿可持续长达24天。血肿消退与局部促炎因子的逐渐减少和抗炎因子的逐渐增加以及新形成血管的增殖和随后的成熟相关。这些事件也与改善的行为结果相关。抗炎细胞因子的表达也抑制了血肿的再吸收。减少下摆。肿瘤大小也与神经功能恢复有关。这些数据表明，血管成熟和抗炎途径可能有助于CSDH的解决和神经恢复。对这两种机制的调控是治疗慢性SDH的潜在靶点。改良的大鼠CSDH模型在我们手中表现出高水平的可重复性，并可能在未来的CSDH研究中是有用的。(C)2015爱思唯尔B.V.保留所有权利。
Chronic subdural hematoma (CSDH) is a common neurological occurrence in the elderly population with significant impact on the quality of life and work. Studies have attempted to determine the risk factors and pathophysiological mechanisms of CSDH using models in numerous mammalian species. To date, these animal models have only been able to reproduce limited durations of hematoma which does not accurately reflect the chronic state of CSDH. To address some of these challenges we modified a rat model of CSDH using two consecutive injections of autologous blood resulting in a hematoma of more than three weeks. We observed inflammatory and angiogenic changes related to the development and recovery of CSDH. In this study the technique for producing a CSDH in a small animal model had a success rate of 78.13%. The hematoma was sustainable up to 24 days. Hematoma resolution was associated with a gradual decrease in local pro-inflammatory factors and gradual increase in anti-inflammatory factors as well as proliferation and subsequent maturation of newly formed vessels. These events were also associated with improved behavioral outcome. Expression of anti-inflammatory cytoldnes also paralleled reabsorption of the hematoma. Reduction in hem. atoma size was also associated with neurological recovery. These data suggest that vessel maturation and anti-inflammatory pathways may contribute to the resolution of CSDH and neurological recovery. The regulation of the two mechanisms is a potential target for the treatment of CSDH. The modified model of rat CSDH demonstrated a high level of reproducibility in our hands and may be useful in future CSDH studies. (C) 2015 Elsevier B.V. All rights reserved.