Isolation of Adult Mouse Cardiomyocytes Using Langendorff Perfusion Apparatus.
Isolation of Adult Mouse Cardiomyocytes Using Langendorff Perfusion Apparatus.
复制标题
使用 Langendorff 灌注装置分离成年小鼠心肌细胞。
DOI：
10.1007/978-1-0716-1480-8_16
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Tong,CarlW
中科院分区：
文献类型：
--
作者：
Liu,Yang;Dostal,DavidE;Tong,CarlW
心脏病是美国的主要原因之一。分离和培养成年心肌细胞对于研究心肌细胞收缩性、心脏肥大和心力衰竭非常重要。与新生儿心肌细胞分离相比，成年小鼠心肌细胞分离具有挑战性，因为心肌细胞之间通过闰盘紧密连接。新产生的转基因小鼠品系的可用性需要建立分离和培养成年小鼠心肌细胞用于体外研究的方案。在这份手稿中，我们描述了一种使用 Langendorff 灌注装置分离成年小鼠心肌细胞的简单方法。简而言之，从成年小鼠中收获心脏并将心脏安装到拉根多夫装置上。在灌注钙消耗和胶原酶消化后，将左心室切碎并过滤。最后，用CaCl2处理分离的心肌细胞。分离的心肌细胞可用于广泛的实验，包括药物筛选。
Heart disease is one of the leading causes of death in the United States. Isolation and culture adult cardiomyocytes are important for studying cardiomyocyte contractility, heart hypertrophy, and cardiac failure. In contrast to neonatal cardiomyocyte isolation, adult mice cardiomyocytes isolation is challenging due to firm connections among cardiomyocytes through intercalated discs. The availability of newly generated genetically modified mouse lines requires to establish protocols to isolation and culture adult mouse cardiomyocyte for in vitro studies. In this manuscript, we described a straightforward method of isolating adult mouse cardiomyocytes using Langendorff perfusion apparatus. Briefly, the hearts were harvested from adult mice and the heart was mounted to Lagendorff apparatus. After perfusion with calcium depletion and collagenase digestion, the left ventricles were minced and filtered. Lastly, the separated cardiomyocytes were treated with CaCl2. The isolated cardiac myocytes can be utilized in a broad range of experiments including screening for drugs.
一种快速技术，用于分离和纯化具有呼吸控制和钙耐受性的成体心肌细胞。
DOI：
--
发表时间：
1976
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
T. Powell;V. Twist
通讯作者：
V. Twist
影响因子：
5
作者：
Louch WE;Sheehan KA;Wolska BM
通讯作者：
Wolska BM