Sexual Dimorphism in Laryngeal Muscle Fibers and Ultrasonic Vocalizations in the Adult Rat
Sexual Dimorphism in Laryngeal Muscle Fibers and Ultrasonic Vocalizations in the Adult Rat
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DOI：
10.1002/lary.26561
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发表时间：
2017-08-01
期刊：
影响因子：
2.6
通讯作者：
Johnson, Aaron M.
中科院分区：
文献类型：
--
作者：
Lenell, Charles;Johnson, Aaron M.
目的：人类的声音在性别上有明显的差异，例如基频和喉部解剖结构的差异，但也有不太明显的差异，例如声音障碍的患病率和类型以及老年人声音变化的表现。然而，在机械动物研究中，很少探讨雄性和雌性之间的差异。本研究的目的是探讨性二型性喉功能和结构的成年大鼠通过检查超声发声声学和肌纤维的大小和类型在甲杓muscle.Study Design：Animal group comparison.Methods：自发超声发声从10个雄性成年大鼠和10个雌性成年大鼠进行了记录，分类，声学分析。甲杓肌的横截面进行染色和成像，以分析肌纤维的大小和类型。声学和肌肉参数进行了统计学比较sexual.Results：雄性大鼠有较低的平均频率短的超声发声。雄性大鼠的甲杓状软骨外侧部的平均纤维尺寸也较大，而发声肌和甲杓状软骨外侧部的整体肌肉面积也较大。然而，肌纤维类型的组成是相似的性别之间的发声和外部分工的甲杓肌。结论：成年雄性和雌性大鼠之间存在功能和结构的喉差异，因此，大鼠模型可以用来进一步研究性别的声音二态性。
Objective: The human voice is sexually dimorphic in obvious ways, such as differences in fundamental frequency and gross laryngeal anatomy, but also in less apparent ways, such as in the prevalence and types of voice disorders and the manifestation of voice changes in advanced age. Differences between males and females are rarely explored, however, in mechanistic animal studies. The goal of this study was to explore sexual dimorphism in laryngeal function and structure in adult rats by examining ultrasonic vocalization acoustics and muscle fiber size and type in the thyroarytenoid muscle.Study Design: Animal group comparison.Methods: Spontaneous ultrasonic vocalizations from 10 male adult rats and 10 female adult rats were recorded, classified, and acoustically analyzed. Cross-sections of the thyroarytenoid muscle were stained and imaged for analysis of muscle fiber size and type. Acoustic and muscle parameters were statistically compared between sexes.Results: Male rats had a lower mean frequency of short ultrasonic vocalizations. Male rats also had a larger mean fiber size in the external division of the thyroarytenoid and larger overall muscle area in both the vocalis and external divisions of the thyroarytenoid. However, muscle fiber type compositions were similar between sexes in both the vocalis and external division of the thyroarytenoid muscles.Conclusion: Functional and structural laryngeal differences exist between adult male and female rats; therefore, the rat model can be used to further study sexual dimorphism of the voice.