Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins
Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins
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DOI：
10.1126/sciadv.aaz6293
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发表时间：
2020-06-01
期刊：
影响因子：
13.6
通讯作者：
Stiel, Andre C.
中科院分区：
文献类型：
--
作者：
Mishra, Kanuj;Stankevych, Mariia;Stiel, Andre C.
我们介绍了两种光致变色蛋白，用于细胞特异性的体内光声成像，并在时间域进行信号分解。我们展示了小鼠身体和大脑中T淋巴细胞、细菌和肿瘤的高度敏感的、多元化的可视化。我们为商业成像系统开发了基于机器学习的软件，用于临时非混合的OA成像，使其能够在生命科学中常规使用。
We introduce two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria, and tumors in the mouse body and brain. We developed machine learning-based software for commercial imaging systems for temporal unmixed OA imaging, enabling its routine use in life sciences.