CHEF ELECTROPHORESIS, A SENSITIVE TECHNIQUE FOR THE DETERMINATION OF DNA DOUBLE-STRAND BREAKS
CHEF ELECTROPHORESIS, A SENSITIVE TECHNIQUE FOR THE DETERMINATION OF DNA DOUBLE-STRAND BREAKS
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DOI：
10.1080/09553008914551591
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发表时间：
1989-10-01
影响因子：
2.6
通讯作者：
ZAJACKOWSKI, J
中科院分区：
文献类型：
--
作者：
BLOCHER, D;EINSPENNER, M;ZAJACKOWSKI, J
钳位均相电场（CHEF）电泳是一种检测中国仓鼠卵巢（CHO）细胞DNA双链断裂的合适方法。它允许分离高达10 Mbp的DNA分子。这种大小的DNA片段的分数与辐射诱导的双链断裂的数量相关。该技术的分辨率极限相当于约1戈伊（伽马射线）的效果。双链断裂修复进行监测照射后，与Co-60 γ射线和修复时间常数确定为t1/2 = 30-35分钟。结合DNA的荧光检测，CHEF电泳提供了一种简单和灵敏的方法，用于确定双链断裂修复，不需要放射性标记的细胞。
It has been demonstrated that clamped homogeneous electrical field (CHEF) electrophoresis is a suitable method for the determination of DNA double-strand breaks in Chinese hamster ovary (CHO) cells. It allows the separation of DNA molecules up to 10 Mbp. The fraction of DNA fragments of this size is correlated with the number of radiation induced double-strand breaks. The resolution limit of the technique is equivalent to the effect of about 1 Gy (gamma-rays). Double-strand break repair was monitored after irradiation with Co-60 gamma rays and the repair time constant determined to t1/2 = 30-35 min. In combination with the detection of DNA by fluorescence, CHEF electrophoresis provides an easy and sensitive method for the determination of double-strand break repair which does not require the radioactive labelling of cells.