DNA barcoding of the endemic New Zealand leafroller moth genera, Ctenopseustis and Planotortrix
DNA barcoding of the endemic New Zealand leafroller moth genera, Ctenopseustis and Planotortrix
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DOI：
10.1111/j.1755-0998.2009.02537.x
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发表时间：
2009-05-01
影响因子：
7.7
通讯作者：
Newcomb, Richard D.
中科院分区：
文献类型：
--
作者：
Langhoff, Pia;Authier, Astrid;Newcomb, Richard D.
分子生物学技术如DNA条形码技术在物种鉴定中的应用越来越广泛，尤其是在隐蔽种复合体的鉴定中。我们已经分析了数据从一个468 bp的线粒体细胞色素氧化酶亚基I（COI）基因从200个标本的12种新西兰特有的卷叶蛾（卷叶蛾科）属Planotorphins和Ctenopseustis的DNA条形码区域是否可以区分这些物种。在分析的200个序列中，回收了72个单倍型，每个属形成一个单独的主要分支。最大似然系统发育方法被用来测试是否物种落入单系分支。结果表明，Ctenopseustis（C.拉加纳角herana，C. filament和C.三个在Planotorphae（P. octo，P. excessana和P. avicenniae）是多系的。Shimodaira-Hasegawa检验拒绝了C.拉加纳角herana、P. octo和P. excessana。同一组的7个物种的序列分歧的物种内和物种之间的水平的比较表明，最大水平的物种内分歧的情况下，大于某些水平的物种间分歧。使用COI基因的该区域的DNA条形码能够区分两个属和每个属内的一些物种;然而，使用该方法不能鉴定许多物种。最后，我们讨论了这种多系性的可能原因，包括不完全的谱系分类、渐渗、水平基因转移和不正确的分类。
Molecular techniques such as DNA barcoding have become popular in assisting species identification especially for cryptic species complexes. We have analysed data from a 468-bp region of the mitochondrial cytochrome oxidase subunit I (COI) gene from 200 specimens of 12 species of endemic New Zealand leafroller moths (Tortricidae) from the genera Planotortrix and Ctenopseustis to assess whether the DNA barcoding region can distinguish these species. Among the 200 sequences analysed, 72 haplotypes were recovered, with each genus forming a separate major clade. Maximum likelihood phylogenetic methods were used to test whether species fell into reciprocally monophyletic clades. The optimal phylogeny showed that four species within the genus Ctenopseustis (C. obliquana, C. herana, C. filicis and C. fraterna) and three within Planotortrix (P. octo, P. excessana and P. avicenniae) are polyphyletic. Shimodaira-Hasegawa tests rejected a null hypothesis of monophyly for the species C. obliquana, C. herana, P. octo and P. excessana. Comparisons of within and between species levels of sequence divergence for the same set of seven species showed cases where maximum levels of within-species divergence were greater than some levels of between-species divergence. DNA barcoding using this region of the COI gene is able to distinguish the two genera and some species within each genus; however, many species cannot be identified using this method. Finally, we discuss the possible reasons for this polyphyly, including incomplete lineage sorting, introgression, horizontal gene transfer and incorrect taxonomy.