Characterization of Colletotrichum isolates from tamarillo, passiflora, and mango in Colombia and identification of a unique species from the genus
Characterization of Colletotrichum isolates from tamarillo, passiflora, and mango in Colombia and identification of a unique species from the genus
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DOI：
10.1094/phyto.2003.93.5.579
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发表时间：
2003-05-01
期刊：
影响因子：
3.2
通讯作者：
Freeman, S
中科院分区：
文献类型：
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作者：
Afanador-Kafuri, L;Minz, D;Freeman, S
本研究旨在鉴定哥伦比亚感染树番茄、芒果和西番莲的炭疽菌物种，并评估宿主物种之间是否发生交叉感染。炭疽菌属 (Colletotrichum spp.) 的分离株通过各种分子方法和形态学标准对来自番茄 (n = 54)、西番莲 (n = 26) 和芒果 (n = 15) 的果实进行了表征。形态学特征将番茄分离物分组为 C. acutatum，将西番莲和芒果分离物分组为 C. gloeosporioides。物种特异性引物分析是可靠的，并确认番茄分离株（除了 Tom-6）为 C. acutatum，芒果分离株（除了 Man-76）为 C. gloeosporioides。然而，西番莲分离株的 DNA 并未通过 C. acutatum 或 C. gloeosporioides 特异性引物扩增，而是与根据这些分离株的内部转录间隔区 (ITS) 1 区域设计的新引物 Col1 发生反应。分离株 Tom-6 和 Man-76 也与 Coll 引物发生阳性反应。所有与尖孢棒杆菌和胶孢棒孢杆菌特异性引物反应的分离株均未能与引物 Coll 反应。从西番莲中分离出的 Pass-35 不与任何分类单元特异性引物发生反应。任意引物聚合酶链式反应 (ap-PCR)、随机扩增聚合酶 DNA (RAPD)-PCR 和富含 A+T 的 DNA 分析将代表性分离株划分为指定物种内的亚组。分子分析表明，C. acutatum tamarillo 分离株是均一的或克隆的，而 C. gloeosporioides mango 分离株和Colletotrichum passiflora 分离株是异质的。同样，完整 ITS (ITS I-5.8S-ITS2) 区域的序列分析鉴定了其各自物种的某些分离株：番茄分离株为 C. acutatum；芒果分离物为 C. gloeosporioides；西番莲、Tom-6 和 Man-76 分离为炭疽菌属 (Colletotrichum sp.)。尚未定义； Pass-35 分离株是一种额外的未定义的炭疽菌属 (Colletotrichum sp)。对西番莲中的炭疽菌、番茄中的 Tom-6 和芒果中的 Man-76 种群进行的分子分析表明，该种群可能不具有宿主特异性。
This study was conducted to identify the species of Colletotrichum infecting tamarillo, mango, and passiflora in Colombia and to assess whether cross-infection between host species is occurring. Isolates of Colletotrichum spp. from tamarillo (n = 54), passiflora (n = 26), and mango (n = 15) were characterized by various molecular methods and by morphological criteria. Morphological characterization grouped the tamarillo isolates as C. acutatum and the passiflora and mango isolates as C. gloeosporioides. Species-specific primer analysis was reliable and confirmed grouping of the tamarillo isolates (besides Tom-6) as C. acutatum and the mango isolates (besides Man-76) as C. gloeosporioides. However, DNA of the passiflora isolates was not amplified by either C. acutatum- or C. gloeosporioides-specific primers, but reacted with a new primer, Col1, designed according to the internal transcribed spacer (ITS) 1 region of these isolates. Isolates Tom-6 and Man-76 also reacted positively with the Coll primer. All the isolates reacting with the C. acutatum- and C. gloeosporioides-specific primers failed to react with primer Coll. Isolate Pass-35 from passiflora did not react with any of the taxon-specific primers. Arbitrarily primed polymerase chain reaction (ap-PCR), random amplified polymerase DNA (RAPD)-PCR, and A+T rich DNA analyses delineated representative isolates into subgroups within the designated species. Molecular analyses indicated that the C. acutatum tamarillo isolates were uniform or clonal, whereas the C. gloeosporioides mango isolates and Colletotrichum passiflora isolates were heterogeneous. Likewise, sequence analysis of the complete ITS (ITS I-5.8S-ITS2) region identified certain isolates to their respective species: tamarillo isolates as C. acutatum; mango isolates as C. gloeosporioides; passiflora, Tom-6, and Man-76 isolates as a Colletotrichum sp. as yet undefined; and the Pass-35 isolate as an additional undefined Colletotrichum sp. Molecular analyses of the population of Colletotrichum isolates from passiflora, Tom-6 from tamarillo, and Man-76 from mango indicate that this population may not be host specific.