The contribution of sucrose metabolism enzymes to sucrose accumulation in Cucumis melo
The contribution of sucrose metabolism enzymes to sucrose accumulation in Cucumis melo
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DOI：
10.21273/jashs.132.5.704
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发表时间：
2007-09-01
影响因子：
1.9
通讯作者：
Schaffer, Arthur A.
中科院分区：
文献类型：
--
作者：
Burger, Yosef;Schaffer, Arthur A.
以7个不同基因型的梅洛L.为材料，研究了甜瓜果实糖积累与蔗糖代谢相关酶活性的关系。覆盖了该物种中发现的蔗糖积累的广泛遗传范围。与蔗糖水平相关的主要决定因素是可溶性酸性转化酶（AI）活性发育损失的遗传变异。只有当AI活性下降到低于实验确定的阈值时，发育中的果实中的蔗糖积累才开始，并持续到从植物中取出果实。蔗糖磷酸合成酶（SPS）、蔗糖合成酶（SuSy）和中性转化酶（NI）活性与蔗糖积累量均呈显著正相关。低蔗糖积累的基因型的特点是低活性的三种酶，无论他们的转化酶活性。最终蔗糖含量最好预测每个基因型的天数水果仍然附着在植物，而其特征在于“蔗糖积累代谢”，其特征主要是AI活性低于阈值，连同SPS，SuSy，NI活动高于阈值水平。
The relationship between sugar accumulation and sucrose metabolism enzyme activities was studied among seven genotypes of Cucumis melo L., covering the broad genetic range of sucrose accumulation found in the species. The primary determinant correlated with sucrose levels was the genetic variation for developmental loss of soluble acid invertase (AI) activity. Sucrose accumulation in the developing fruit began only when AI activity declined to less than an experimentally determined threshold value, and continued until removal of the fruit from the plant. In addition, the activities of sucrose phosphate synthase (SPS), sucrose synthase (SuSy), and neutral invertase (NI) were all positively correlated with sucrose accumulation among the genotypes. The low-sucrose-accumulating genotypes were characterized by low activities of each of the three enzymes, irrespective of their invertase activities. Final sucrose content was best predicted for each genotype by the number of days the fruit remained attached to the plant while characterized by "sucrose accumulation metabolism," which was characterized primarily by AI activity less than threshold values, together with SPS, SuSy, and NI activities higher than threshold levels.