Defensin from disk abalone Haliotis discus discus: Molecular cloning, sequence characterization and immune response against bacterial infection
Defensin from disk abalone Haliotis discus discus: Molecular cloning, sequence characterization and immune response against bacterial infection
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DOI：
10.1016/j.fsi.2009.11.005
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发表时间：
2010-02-01
影响因子：
4.7
通讯作者：
Lee, Jehee
中科院分区：
文献类型：
--
作者：
De Zoysa, Mahanama;Whang, Ilson;Lee, Jehee
基因编码的抗菌肽（AMP）在宿主抵抗病原体的防御系统中起着重要作用。在这项研究中，一个新的软体动物防御素的cDNA从一个标准化的cDNA文库构建从盘鲍的整个组织。鲍防御素前体（pro-defensin）编码区全长198 bp，由66个氨基酸组成，成熟的防御素由48个氨基酸组成。鲍鱼防御素具有一个无脊椎动物防御素家族结构域、六个半胱氨酸残基及其二硫键以C-1-C-4、C-2-C-5和C-3-C-6的形式排列、一个α螺旋的三维结构和系统发育关系，表明鲍鱼防御素可能是无脊椎动物防御素家族的一个新成员，并与节肢动物防御素有一定的亲缘关系。在未受刺激的鲍鱼中，防御素转录本组成型表达于所有检测的组织，包括血细胞、鳃、外套膜、肌肉、消化道和肝胰腺。结果表明，溶藻弧菌、副溶血弧菌和单核细胞增多性李斯特菌混合感染后不同时间，鲍鱼血细胞、鳃和消化道中的防御素转录水平均显著升高。我们的总体结果表明，盘鲍防御素可能参与免疫应答反应，作为宿主防御病原菌。(C)2009爱思唯尔有限公司保留所有权利。
Gene-encoded antimicrobial peptides (AMPs) serve a major role in host defense systems against pathogens. In this study, cDNA of a new mollusk defensin was identified from a normalized cDNA library constructed from whole tissues of disk abalone. Abalone defensin peptide (pro-defensin) has a 198-bp coding sequence comprised of a putative 66 amino acids with a mature defensin consisting of 48 amino acid residues. The presence of an invertebrate defensin family domain, an arrangement of six cysteine residues and their disulfide linkage in C-1-C-4, C-2-C-5 and C-3-C-6 form, an alpha helix in three-dimensional structure and a phylogenetic relationship suggests that abalone defensin could be a new member of the invertebrate defensin family, and related to arthropod defensins. In non-stimulated abalone, defensin transcripts were constitutively expressed in all examined tissues including hemocytes, gills, mantle, muscle, digestive tract and hepatopancreas. It was observed that abalone defensin transcripts were significantly induced in hemocytes, gills and digestive tract at different time intervals after infection by pathogenic bacteria mixture containing Vibrio alginolyticus, Vibrio parahemolyticus and Lysteria monocytogenes. Our overall results suggest that disk abalone defensin could be involved in the immune response reactions as a host defense against pathogenic bacteria. (C) 2009 Elsevier Ltd. All rights reserved.